Catechin Attenuates the Effect of Combined Arsenic and Deltamethrin Toxicity by Abrogation of Oxidative Stress and Inflammation in Wistar Rats

Afolabi Olusegun Kayode; Arinola Abimbola; Aderibigbe Felix Adesola; Folarin Dasola Teslim; Wusu Adedoja Dorcas

doi:doi:10.11648/j.ab.20190702.12

| Peer-Reviewed

Catechin Attenuates the Effect of Combined Arsenic and Deltamethrin Toxicity by Abrogation of Oxidative Stress and Inflammation in Wistar Rats

Afolabi Olusegun Kayode, Arinola Abimbola, Aderibigbe Felix Adesola, Folarin Dasola Teslim, Wusu Adedoja Dorcas

Published in Advances in Biochemistry (Volume 7, Issue 2)

Received: 27 July 2019 Accepted: 18 August 2019 Published: 26 August 2019

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

This study was aimed at evaluating the protective role of catechin (CT) against toxicity induced by combined exposure to arsenic (As) and deltamethrin (DM) in rats. Thirty-five (35) male Wistar rats were divided into 5 groups of 7 animals each. Treatment of each group was as follows: Control (C) administered corn oil (1ml kg^-1), catechin only (CT) at 40mg kg^-1, As+DM administered As (100ppm) in their drinking water and DM at a dose of 7.5mg kg^-1 (1/20^th LD₅₀), As+DM-CT₄₀ treated as As+DM in addition to oral administration of CT at 40mg kg^-1 while the last group, As+DM-CT₈₀ received the same treatment as As+DM, along with oral CT treatment at a dose of 80mg kg^-1. The treatment lasted for 28 days. Effect of the treatment in inducing oxidative damage was appraised by estimating levels of lipid peroxidation, protein oxidation, glutathione and total antioxidant capacity in the liver, kidney, and testis of the rats. Also, the activities of superoxide dismutase, catalase, and glutathione peroxidase were assayed in the tissues. For the evaluation of inflammation, plasma levels of interleukin-6, tumor necrosis factor-alpha and 8-nitroguanine were determined. The result showed that the combination of As and DM gave rise to marked alterations of these parameters but supplementation with CT attenuated these effects.

Published in	Advances in Biochemistry (Volume 7, Issue 2)
DOI	10.11648/j.ab.20190702.12
Page(s)	51-58
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Arsenic, Deltamethrin, Oxidative Stress, Inflammation, Catechin

References

[1]	Tchounwou PB, Yedjou CG, Patlolla AK, Sutton DJ. Heavy metal toxicity and the environment. Molecular, clinical and environmental toxicology: Springer; 2012. p. 133-64.
[2]	Zikankuba VL, Mwanyika G, Ntwenya JE, James A. Pesticide regulations and their malpractice implications on food and environment safety. Cogent Food & Agriculture 2019; 5: 1601544.
[3]	ATSDR U. Toxicological Profile for Arsenic. Agency for Toxic Substances and Disease Registry. US Department of Health and Human Services, Centers for Disease Control and Prevention; 2007.
[4]	Flora S, Dwivedi N, Deb U, Kushwaha P, Lomash V. Effects of co-exposure to arsenic and dichlorvos on glutathione metabolism, neurological, hepatic variables and tissue histopathology in rats. Toxicology Research 2014; 3: 23-31.
[5]	Hughes MF, Beck BD, Chen Y, Lewis AS, Thomas DJ. Arsenic exposure and toxicology: a historical perspective. Toxicological Sciences 2011; 123: 305-32.
[6]	Mazumder DG, Dasgupta U. Chronic arsenic toxicity: studies in West Bengal, India. The Kaohsiung journal of medical sciences 2011; 27: 360-70.
[7]	Peters BA, Liu X, Hall MN, Ilievski V, Slavkovich V, Siddique AB, et al. Arsenic exposure, inflammation, and renal function in Bangladeshi adults: effect modification by plasma glutathione redox potential. Free Radical Biology and Medicine 2015; 85: 174-82.
[8]	Jomova K, Jenisova Z, Feszterova M, Baros S, Liska J, Hudecova D, et al. Arsenic: toxicity, oxidative stress and human disease. Journal of Applied Toxicology 2011; 31: 95-107.
[9]	Debnath M, Khan MS. Health concerns of pesticides. Pesticide Residue in Foods: Springer; 2017. p. 103-18.
[10]	Wani WY, Gudup S, Sunkaria A, Bal A, Singh PP, Kandimalla RJ, et al. Protective efficacy of mitochondrial targeted antioxidant MitoQ against dichlorvos induced oxidative stress and cell death in rat brain. Neuropharmacology 2011; 61: 1193-201.
[11]	Khan AM, Raina R, Dubey N, Verma PK. Effect of deltamethrin and fluoride co-exposure on the brain antioxidant status and cholinesterase activity in Wistar rats. Drug and chemical toxicology 2018; 41: 123-7.
[12]	Dubey N, Khan AM, Raina R. Sub-acute deltamethrin and fluoride toxicity induced hepatic oxidative stress and biochemical alterations in rats. Bulletin of environmental contamination and toxicology 2013; 91: 334-8.
[13]	Dubey N, Raina R, Khan AM. Toxic effects of deltamethrin and fluoride on antioxidant parameters in rats. Fluoride 2012; 45: 242-6.
[14]	Ncir M, Saoudi M, Sellami H, Rahmouni F, Lahyani A, Makni Ayadi F, et al. In vitro and in vivo studies of Allium sativum extract against deltamethrin-induced oxidative stress in rats brain and kidney. Archives of physiology and biochemistry 2018; 124: 207-17.
[15]	Ogaly HA, Khalaf A, Ibrahim MA, Galal MK, Abd-Elsalam RM. Influence of green tea extract on oxidative damage and apoptosis induced by deltamethrin in rat brain. Neurotoxicology and teratology 2015; 50: 23-31.
[16]	Pace C, Dagda R, Angermann J. Antioxidants protect against arsenic induced mitochondrial cardio-toxicity. Toxics 2017; 5: 38.
[17]	Sárközi K, Papp A, Máté Z, Horváth E, Paulik E, Szabó A. Rutin, a flavonoid phytochemical, ameliorates certain behavioral and electrophysiological alterations and general toxicity of oral arsenic in rats. Acta biologica hungarica 2015; 66: 14-26.
[18]	Samarghandian S, Azimi-Nezhad M, Farkhondeh T. Catechin treatment ameliorates diabetes and its complications in streptozotocin-induced diabetic rats. Dose-Response 2017; 15: 1559325817691158.
[19]	Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. Journal of biological chemistry 1951; 193: 265-75.
[20]	Buege JA, Aust SD. Microsomal lipid peroxidation. Methods in enzymology: Elsevier; 1978. p. 302-10.
[21]	Nouroozzadeh J, Tajaddinisarmadi J, Wolff SP. Measurement of plasma hydroperoxide concentrations by the ferrous oxidation-xylenol orange assay in conjunction with triphenylphosphine. Analytical biochemistry 1994; 220: 403-9.
[22]	Witko-Sarsat V, Friedlander M, Capeillère-Blandin C, Nguyen-Khoa T, Nguyen AT, Zingraff J, et al. Advanced oxidation protein products as a novel marker of oxidative stress in uremia. Kidney international 1996; 49: 1304-13.
[23]	Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Analytical biochemistry 1996; 239: 70-6.
[24]	Moron MS, Depierre JW, Mannervik B. Levels of glutathione, glutathione reductase and glutathione S-transferase activities in rat lung and liver. Biochimica et Biophysica Acta (BBA)-General Subjects 1979; 582: 67-78.
[25]	Aebi H. Catalase. Methods of enzymatic analysis: Elsevier; 1974. p. 673-84.
[26]	Misra HP, Fridovich I. The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase. Journal of Biological chemistry 1972; 247: 3170-5.
[27]	Rotruck JT, Pope AL, Ganther HE, Swanson A, Hafeman DG, Hoekstra W. Selenium: biochemical role as a component of glutathione peroxidase. Science 1973; 179: 588-90.
[28]	Singh N, Gupta VK, Kumar A, Sharma B. Synergistic effects of heavy metals and pesticides in living systems. Frontiers in chemistry 2017; 5: 70.
[29]	Adamkovicova M, Toman R, Cabaj M, Massanyi P, Martiniakova M, Omelka R, et al. Effects of subchronic exposure to cadmium and diazinon on testis and epididymis in rats. The Scientific World Journal 2014; 2014.
[30]	Pachauri V, Mehta A, Mishra D, Flora SJ. Arsenic induced neuronal apoptosis in guinea pigs is Ca₂₊ dependent and abrogated by chelation therapy: role of voltage gated calcium channels. Neurotoxicology 2013; 35: 137-45.
[31]	Uchendu C, Ambali SF, Ayo JO, Esievo KA. The protective role of alpha-lipoic acid on long-term exposure of rats to the combination of chlorpyrifos and deltamethrin pesticides. Toxicology and industrial health 2017; 33: 159-70.
[32]	Afolabi OK, Wusu AD, Ugbaja R, Fatoki JO. Aluminium phosphide-induced testicular toxicity through oxidative stress in Wistar rats: Ameliorative role of hesperidin. Toxicology Research and Application 2018; 2: 2397847318812794.
[33]	Oliveira JM, Losano NF, Condessa SS, de Freitas RMP, Cardoso SA, Freitas MB, et al. Exposure to deltamethrin induces oxidative stress and decreases of energy reserve in tissues of the Neotropical fruit-eating bat Artibeus lituratus. Ecotoxicology and environmental safety 2018; 148: 684-92.
[34]	Saoudi M, Badraoui R, Bouhajja H, Ncir M, Rahmouni F, Grati M, et al. Deltamethrin induced oxidative stress in kidney and brain of rats: Protective effect of Artemisia campestris essential oil. Biomedicine & Pharmacotherapy 2017; 94: 955-63.
[35]	Altikat S, Uysal K, Kuru HI, Kavasoglu M, Ozturk GN, Kucuk A. The effect of arsenic on some antioxidant enzyme activities and lipid peroxidation in various tissues of mirror carp (Cyprinus carpio carpio). Environmental Science and Pollution Research 2015; 22: 3212-8.
[36]	Bashandy SA, El Awdan SA, Ebaid H, Alhazza IM. Antioxidant potential of Spirulina platensis mitigates oxidative stress and reprotoxicity induced by sodium arsenite in male rats. Oxidative medicine and cellular longevity 2016; 2016.
[37]	Demir F, Uzun FG, Durak D, Kalender Y. Subacute chlorpyrifos-induced oxidative stress in rat erythrocytes and the protective effects of catechin and quercetin. Pesticide Biochemistry and Physiology 2011; 99: 77-81.
[38]	El-Desoky GE, Bashandy SA, Alhazza IM, Al-Othman ZA, Aboul-Soud MA, Yusuf K. Improvement of mercuric chloride-induced testis injuries and sperm quality deteriorations by Spirulina platensis in rats. PLoS One 2013; 8: e59177.
[39]	Dwivedi N, Flora G, Kushwaha P, Flora SJ. Alpha-lipoic acid protects oxidative stress, changes in cholinergic system and tissue histopathology during co-exposure to arsenic-dichlorvos in rats. Environmental toxicology and pharmacology 2014; 37: 7-23.
[40]	Dwivedi N, Flora SJ. Concomitant exposure to arsenic and organophosphates on tissue oxidative stress in rats. Food and chemical toxicology 2011; 49: 1152-9.
[41]	Yousefi B, Ahmadi Y, Ghorbanihaghjo A, Faghfoori Z. Serum arsenic and lipid peroxidation levels in patients with multiple sclerosis. Biological trace element research 2014; 158: 276-9.
[42]	Sharma DK, Ansari BA. Effects of deltamethrin on CAT, LPO and GSH in tissues of zebra fish Danio rerio. Research Journal of Environmental Toxicology 2013; 7: 38-46.
[43]	El-Aziz TAA, Mohamed RH, Pasha HF, Abdel-Aziz HR. Catechin protects against oxidative stress and inflammatory-mediated cardiotoxicity in adriamycin-treated rats. Clinical and experimental medicine 2012; 12: 233-40.
[44]	Fan F-Y, Sang L-X, Jiang M. Catechins and their therapeutic benefits to inflammatory bowel disease. Molecules 2017; 22: 484.
[45]	Mehra P, Garg M, Koul A, Bansal DD. Effect of (+)-catechin hydrate on oxidative stress induced by high sucrose and high fat diet in male Wistar rats. 2013.
[46]	Forrester SJ, Kikuchi DS, Hernandes MS, Xu Q, Griendling KK. Reactive oxygen species in metabolic and inflammatory signaling. Circulation Research 2018; 122: 877-902.
[47]	Arslan H, Altun S, Özdemir S. Acute toxication of deltamethrin results in activation of iNOS, 8-OHdG and up-regulation of caspase 3, iNOS gene expression in common carp (Cyprinus carpio L.). Aquatic Toxicology 2017; 187: 90-9.
[48]	Hiraku Y, Sakai K, Shibata E, Kamijima M, Hisanaga N, Ma N, et al. Formation of the Nitrative DNA Lesion 8-nitroguanine is Associated with Asbestos Contents in Human Lung Tissues: A Pilot Atudy. Journal of occupational health 2014: 13-0231-OA.

Cite This Article

Plain Text BibTeX RIS

APA Style

Afolabi Olusegun Kayode, Arinola Abimbola, Aderibigbe Felix Adesola, Folarin Dasola Teslim, Wusu Adedoja Dorcas. (2019). Catechin Attenuates the Effect of Combined Arsenic and Deltamethrin Toxicity by Abrogation of Oxidative Stress and Inflammation in Wistar Rats. Advances in Biochemistry, 7(2), 51-58. https://doi.org/10.11648/j.ab.20190702.12

Copy | Download

ACS Style

Afolabi Olusegun Kayode; Arinola Abimbola; Aderibigbe Felix Adesola; Folarin Dasola Teslim; Wusu Adedoja Dorcas. Catechin Attenuates the Effect of Combined Arsenic and Deltamethrin Toxicity by Abrogation of Oxidative Stress and Inflammation in Wistar Rats. Adv. Biochem. 2019, 7(2), 51-58. doi: 10.11648/j.ab.20190702.12

Copy | Download

AMA Style

Afolabi Olusegun Kayode, Arinola Abimbola, Aderibigbe Felix Adesola, Folarin Dasola Teslim, Wusu Adedoja Dorcas. Catechin Attenuates the Effect of Combined Arsenic and Deltamethrin Toxicity by Abrogation of Oxidative Stress and Inflammation in Wistar Rats. Adv Biochem. 2019;7(2):51-58. doi: 10.11648/j.ab.20190702.12

Copy | Download

@article{10.11648/j.ab.20190702.12,
  author = {Afolabi Olusegun Kayode and Arinola Abimbola and Aderibigbe Felix Adesola and Folarin Dasola Teslim and Wusu Adedoja Dorcas},
  title = {Catechin Attenuates the Effect of Combined Arsenic and Deltamethrin Toxicity by Abrogation of Oxidative Stress and Inflammation in Wistar Rats},
  journal = {Advances in Biochemistry},
  volume = {7},
  number = {2},
  pages = {51-58},
  doi = {10.11648/j.ab.20190702.12},
  url = {https://doi.org/10.11648/j.ab.20190702.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ab.20190702.12},
  abstract = {This study was aimed at evaluating the protective role of catechin (CT) against toxicity induced by combined exposure to arsenic (As) and deltamethrin (DM) in rats. Thirty-five (35) male Wistar rats were divided into 5 groups of 7 animals each. Treatment of each group was as follows: Control (C) administered corn oil (1ml kg-1), catechin only (CT) at 40mg kg-1, As+DM administered As (100ppm) in their drinking water and DM at a dose of 7.5mg kg-1 (1/20th LD50), As+DM-CT40 treated as As+DM in addition to oral administration of CT at 40mg kg-1 while the last group, As+DM-CT80 received the same treatment as As+DM, along with oral CT treatment at a dose of 80mg kg-1. The treatment lasted for 28 days. Effect of the treatment in inducing oxidative damage was appraised by estimating levels of lipid peroxidation, protein oxidation, glutathione and total antioxidant capacity in the liver, kidney, and testis of the rats. Also, the activities of superoxide dismutase, catalase, and glutathione peroxidase were assayed in the tissues. For the evaluation of inflammation, plasma levels of interleukin-6, tumor necrosis factor-alpha and 8-nitroguanine were determined. The result showed that the combination of As and DM gave rise to marked alterations of these parameters but supplementation with CT attenuated these effects.},
 year = {2019}
}

Copy | Download

TY  - JOUR
T1  - Catechin Attenuates the Effect of Combined Arsenic and Deltamethrin Toxicity by Abrogation of Oxidative Stress and Inflammation in Wistar Rats
AU  - Afolabi Olusegun Kayode
AU  - Arinola Abimbola
AU  - Aderibigbe Felix Adesola
AU  - Folarin Dasola Teslim
AU  - Wusu Adedoja Dorcas
Y1  - 2019/08/26
PY  - 2019
N1  - https://doi.org/10.11648/j.ab.20190702.12
DO  - 10.11648/j.ab.20190702.12
T2  - Advances in Biochemistry
JF  - Advances in Biochemistry
JO  - Advances in Biochemistry
SP  - 51
EP  - 58
PB  - Science Publishing Group
SN  - 2329-0862
UR  - https://doi.org/10.11648/j.ab.20190702.12
AB  - This study was aimed at evaluating the protective role of catechin (CT) against toxicity induced by combined exposure to arsenic (As) and deltamethrin (DM) in rats. Thirty-five (35) male Wistar rats were divided into 5 groups of 7 animals each. Treatment of each group was as follows: Control (C) administered corn oil (1ml kg-1), catechin only (CT) at 40mg kg-1, As+DM administered As (100ppm) in their drinking water and DM at a dose of 7.5mg kg-1 (1/20th LD50), As+DM-CT40 treated as As+DM in addition to oral administration of CT at 40mg kg-1 while the last group, As+DM-CT80 received the same treatment as As+DM, along with oral CT treatment at a dose of 80mg kg-1. The treatment lasted for 28 days. Effect of the treatment in inducing oxidative damage was appraised by estimating levels of lipid peroxidation, protein oxidation, glutathione and total antioxidant capacity in the liver, kidney, and testis of the rats. Also, the activities of superoxide dismutase, catalase, and glutathione peroxidase were assayed in the tissues. For the evaluation of inflammation, plasma levels of interleukin-6, tumor necrosis factor-alpha and 8-nitroguanine were determined. The result showed that the combination of As and DM gave rise to marked alterations of these parameters but supplementation with CT attenuated these effects.
VL  - 7
IS  - 2
ER  -

Copy | Download

Author Information

Afolabi Olusegun Kayode

Department of Biochemistry, Faculty of Basic Medical Sciences, Ladoke Akintola University of Technology, Ogbomoso, Nigeria
Arinola Abimbola

Department of Biochemistry, Faculty of Basic Medical Sciences, Ladoke Akintola University of Technology, Ogbomoso, Nigeria
Aderibigbe Felix Adesola

Department of Biochemistry, Faculty of Basic Medical Sciences, Ladoke Akintola University of Technology, Ogbomoso, Nigeria
Folarin Dasola Teslim

Department of Biochemistry, Faculty of Basic Medical Sciences, Ladoke Akintola University of Technology, Ogbomoso, Nigeria
Wusu Adedoja Dorcas

Department of Biochemistry, Faculty of Science, Lagos State University, Ojo, Lagos

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Afolabi Olusegun Kayode, Arinola Abimbola, Aderibigbe Felix Adesola, Folarin Dasola Teslim, Wusu Adedoja Dorcas. (2019). Catechin Attenuates the Effect of Combined Arsenic and Deltamethrin Toxicity by Abrogation of Oxidative Stress and Inflammation in Wistar Rats. Advances in Biochemistry, 7(2), 51-58. https://doi.org/10.11648/j.ab.20190702.12

Copy | Download

ACS Style

Afolabi Olusegun Kayode; Arinola Abimbola; Aderibigbe Felix Adesola; Folarin Dasola Teslim; Wusu Adedoja Dorcas. Catechin Attenuates the Effect of Combined Arsenic and Deltamethrin Toxicity by Abrogation of Oxidative Stress and Inflammation in Wistar Rats. Adv. Biochem. 2019, 7(2), 51-58. doi: 10.11648/j.ab.20190702.12

Copy | Download

AMA Style

Afolabi Olusegun Kayode, Arinola Abimbola, Aderibigbe Felix Adesola, Folarin Dasola Teslim, Wusu Adedoja Dorcas. Catechin Attenuates the Effect of Combined Arsenic and Deltamethrin Toxicity by Abrogation of Oxidative Stress and Inflammation in Wistar Rats. Adv Biochem. 2019;7(2):51-58. doi: 10.11648/j.ab.20190702.12

Copy | Download

@article{10.11648/j.ab.20190702.12,
  author = {Afolabi Olusegun Kayode and Arinola Abimbola and Aderibigbe Felix Adesola and Folarin Dasola Teslim and Wusu Adedoja Dorcas},
  title = {Catechin Attenuates the Effect of Combined Arsenic and Deltamethrin Toxicity by Abrogation of Oxidative Stress and Inflammation in Wistar Rats},
  journal = {Advances in Biochemistry},
  volume = {7},
  number = {2},
  pages = {51-58},
  doi = {10.11648/j.ab.20190702.12},
  url = {https://doi.org/10.11648/j.ab.20190702.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ab.20190702.12},
  abstract = {This study was aimed at evaluating the protective role of catechin (CT) against toxicity induced by combined exposure to arsenic (As) and deltamethrin (DM) in rats. Thirty-five (35) male Wistar rats were divided into 5 groups of 7 animals each. Treatment of each group was as follows: Control (C) administered corn oil (1ml kg-1), catechin only (CT) at 40mg kg-1, As+DM administered As (100ppm) in their drinking water and DM at a dose of 7.5mg kg-1 (1/20th LD50), As+DM-CT40 treated as As+DM in addition to oral administration of CT at 40mg kg-1 while the last group, As+DM-CT80 received the same treatment as As+DM, along with oral CT treatment at a dose of 80mg kg-1. The treatment lasted for 28 days. Effect of the treatment in inducing oxidative damage was appraised by estimating levels of lipid peroxidation, protein oxidation, glutathione and total antioxidant capacity in the liver, kidney, and testis of the rats. Also, the activities of superoxide dismutase, catalase, and glutathione peroxidase were assayed in the tissues. For the evaluation of inflammation, plasma levels of interleukin-6, tumor necrosis factor-alpha and 8-nitroguanine were determined. The result showed that the combination of As and DM gave rise to marked alterations of these parameters but supplementation with CT attenuated these effects.},
 year = {2019}
}

Copy | Download

TY  - JOUR
T1  - Catechin Attenuates the Effect of Combined Arsenic and Deltamethrin Toxicity by Abrogation of Oxidative Stress and Inflammation in Wistar Rats
AU  - Afolabi Olusegun Kayode
AU  - Arinola Abimbola
AU  - Aderibigbe Felix Adesola
AU  - Folarin Dasola Teslim
AU  - Wusu Adedoja Dorcas
Y1  - 2019/08/26
PY  - 2019
N1  - https://doi.org/10.11648/j.ab.20190702.12
DO  - 10.11648/j.ab.20190702.12
T2  - Advances in Biochemistry
JF  - Advances in Biochemistry
JO  - Advances in Biochemistry
SP  - 51
EP  - 58
PB  - Science Publishing Group
SN  - 2329-0862
UR  - https://doi.org/10.11648/j.ab.20190702.12
AB  - This study was aimed at evaluating the protective role of catechin (CT) against toxicity induced by combined exposure to arsenic (As) and deltamethrin (DM) in rats. Thirty-five (35) male Wistar rats were divided into 5 groups of 7 animals each. Treatment of each group was as follows: Control (C) administered corn oil (1ml kg-1), catechin only (CT) at 40mg kg-1, As+DM administered As (100ppm) in their drinking water and DM at a dose of 7.5mg kg-1 (1/20th LD50), As+DM-CT40 treated as As+DM in addition to oral administration of CT at 40mg kg-1 while the last group, As+DM-CT80 received the same treatment as As+DM, along with oral CT treatment at a dose of 80mg kg-1. The treatment lasted for 28 days. Effect of the treatment in inducing oxidative damage was appraised by estimating levels of lipid peroxidation, protein oxidation, glutathione and total antioxidant capacity in the liver, kidney, and testis of the rats. Also, the activities of superoxide dismutase, catalase, and glutathione peroxidase were assayed in the tissues. For the evaluation of inflammation, plasma levels of interleukin-6, tumor necrosis factor-alpha and 8-nitroguanine were determined. The result showed that the combination of As and DM gave rise to marked alterations of these parameters but supplementation with CT attenuated these effects.
VL  - 7
IS  - 2
ER  -

Copy | Download