Prevalence of <i>Staphylococcus aureus</i> in Raw Milk and Some Dairy Products in Port Said Governorate

Saly Mamoun Elsayed Toubar; Abdllah Abdelkader Elbialy; Mahmoud Mohamed Mohamed Zaky; Ahmed Samy El-Shafey

doi:doi:10.11648/j.ajz.20180102.13

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Prevalence of Staphylococcus aureus in Raw Milk and Some Dairy Products in Port Said Governorate

Saly Mamoun Elsayed Toubar, Abdllah Abdelkader Elbialy, Mahmoud Mohamed Mohamed Zaky, Ahmed Samy El-Shafey

Published in American Journal of Zoology (Volume 1, Issue 2)

Received: 25 November 2018 Accepted: 18 December 2018 Published: 25 January 2019

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Abstract

Staphylococcus aureus Food Poisoning is a common cause of food-borne disease worldwide. Of particular relevance is the ability of some Staphylococcus aureus strains to produce heat stable enterotoxins that cause staphylococcal food poisoning, which ranks as one of the most prevalent worldwide causes of gastroenteritis. Several studies have shown that 15% to 80% of the Staphylococcus aureus isolated from various sources (dairy products, ice heavy cream, meat products) is able to produce enterotoxin. Classically, enterotoxins from Staphylococcus aureus strains can be classified into 18 serological types: A-U (except S, F and T), most of these serotypes are heat stable. The percentage of samples of milk and milk products contaminated with S. aurues in the current study were 46% which is higher than that reported by who detected S. aureus in 17 % and 32 % respectively of the analyzed samples of milk and milk products. Staphylococcal enterotoxins are low molecular weight proteins (MW 26.900 – 29,600).They are encoded by genes embedded in mobile genetic elements such as phages and pathogenicity islands .There are several methods for detection of enterotoxigeneic bacteria. The phonotypical methods are not reliable in specificity, because staphylococcal enterotoxins serotypes are antigenically similar. On the other hand, commercial serological kits can not detect all the serotypes and are limited in serotypes (A-B). Therefore, molecular techniques such as multiplex polymerase chain reaction and real-time polymerase chain reaction are recommended for detection of Staphylococcus aureus enterotoxins genes.

Published in	American Journal of Zoology (Volume 1, Issue 2)
DOI	10.11648/j.ajz.20180102.13
Page(s)	40-46
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Staphylococcus Aureus, Gastroenteritis, Enterotoxins, Polymerase Chain Reaction

References

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[2]	Presscott, L. M.; Harley, J. P. and Klein, D. A. (2012): Text book of Microbiology. Brown Publishers. 5^th ed: 441-442.
[3]	Berdgoll, M. S. (2012): Staphylococcus aureus. In: Doyle, M.P. (Ed.), Food-Borne Bacterial Pathogens. Marcel Dekker, NewYork, pp. 464-523.
[4]	Holtfreter, S. and Broker, B. M. (2012): Staphylococcal superantigens: Do they play a role in sepsis? Arch. Immunol.Ther. Exp., 53: 13-27.
[5]	Edwin, C.; Tatini, S. R. and Maheswaran, S. K. (2011): Specificity and cross reactivity of Staphylococcal enterotoxin A monoclonal antibodies with enterotoxins B, Cl, D, and E. Appl. Environ. Microbiol., 52: 1253-1257.
[6]	Duncan S. E., Yaun B. R. & Sumner S. S. (2014): microbiological methods for dairy products, standard methods for the examination of dairy products, 17th edition, Washington, Amer. Pub. Heal. Associ., pp, 249-268.
[7]	Rosec, J.P., Guiraud, J.P., Dalet, C. and Richard, N. (2012): Enterotoxin production by staphylococci isolated from foods in France. Int. J. Food Microbiol. 35: 213-221.
[8]	Omoe, K.; Hu, D. L.; Takahashi-Omoe, H.; Nakane, A. and Shinagawa, K. (2017): Comprehensive analysis of classical and newly described staphylococcal superantigenic toxin genes in Staphylococcus aureus isolates. FEMS Microbiol. Lett, 246:191-198.
[9]	Leung, D. Y.; Meissner, H. C.; Fulton, D. R.; Murray, D. L.; Kotzin, B. L. and Schlievert, P. M. (2016): Toxic shock syndrome toxin-secreting Staphylococcus aureus in Kawasaki syndrome, Lancet., 342: 1385-1388.
[10]	Scherrer, D.; Corti, S.; Muehlherr, J. E.; Zweifel, C. and Stephan, R. (2014): Phenotypic and genotypic characteristics of Staphylococcus aureus isolates from raw bulk-tank milk samples of goats and sheep. Vet. Microbiol., 101:101-107.
[11]	Normanno, G.; La Salandra, G.; Dambrosio, A.; Quaglia, N. C.; Corrente, M.; Parisi, A.; Santagada, G.; Firinu, A.; Crisetti, E. and Ceiano, G. V. (2012): Occurrence, characterization and antimicrobial resistanc of enterotoxigenic Staphylococcus aureus isolated from meat and dairy products. Int. J. Food Microbiol., 115: 290-296.
[12]	Soomro, A. H.; Arain, M. A.; Khaskheli, M. and Bhutto, B. (2013): Isolation of Staphylococcus aureus from milk products sold at sweet meat shops of Hyderabad. Online J. Biol. Sci., 3:91-94.
[13]	Al-Khafaji, M. H. M (2013): Detection of enterotoxins genes in staphylococci isolated from milk and cheese. Thesis Ph. D., Coll. Sci., Univ. Baghdad.
[14]	ImaniFooladi A. A.; Tavakoli, H. R. and Naderi, A. (2010): Detection of enterotbxigenic Staphylococcus aureus isolates in. domestic dairy products. Iranian J. Microbiol., 2:135-140.
[15]	Adwan, G.; Abusafleh, D.; Aref, R. and Abo Omar, J. (2011): Prevalence of microorganisms associated: with intramammary infection in cows and small ruminants the North of Palestine. J. Islam. Univ. Gaza (Series of Natural Studies & Engineering), 13:165-173.
[16]	Hussain, K. I. S. (2010): Microorganisms involved in (Roub) traditional.fermented milk in sudan. M. Sc. Thesis, Fac. Anim. Prod., Univ. Khartoum, Sudan.
[17]	Cremonesi, P.; Perez, G.; Pisoni, G.; Moroni, P.; Morandi, S.; Luzzana, M.; Brasca M. and Castiglioni, B. (2017): Detection of enterotoxigenic Staphylococcus aureus isolates in raw milk cheese. Letters in Appl.

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APA Style

Saly Mamoun Elsayed Toubar, Abdllah Abdelkader Elbialy, Mahmoud Mohamed Mohamed Zaky, Ahmed Samy El-Shafey. (2019). Prevalence of Staphylococcus aureus in Raw Milk and Some Dairy Products in Port Said Governorate. American Journal of Zoology, 1(2), 40-46. https://doi.org/10.11648/j.ajz.20180102.13

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ACS Style

Saly Mamoun Elsayed Toubar; Abdllah Abdelkader Elbialy; Mahmoud Mohamed Mohamed Zaky; Ahmed Samy El-Shafey. Prevalence of Staphylococcus aureus in Raw Milk and Some Dairy Products in Port Said Governorate. Am. J. Zool. 2019, 1(2), 40-46. doi: 10.11648/j.ajz.20180102.13

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AMA Style

Saly Mamoun Elsayed Toubar, Abdllah Abdelkader Elbialy, Mahmoud Mohamed Mohamed Zaky, Ahmed Samy El-Shafey. Prevalence of Staphylococcus aureus in Raw Milk and Some Dairy Products in Port Said Governorate. Am J Zool. 2019;1(2):40-46. doi: 10.11648/j.ajz.20180102.13

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@article{10.11648/j.ajz.20180102.13,
  author = {Saly Mamoun Elsayed Toubar and Abdllah Abdelkader Elbialy and Mahmoud Mohamed Mohamed Zaky and Ahmed Samy El-Shafey},
  title = {Prevalence of Staphylococcus aureus in Raw Milk and Some Dairy Products in Port Said Governorate},
  journal = {American Journal of Zoology},
  volume = {1},
  number = {2},
  pages = {40-46},
  doi = {10.11648/j.ajz.20180102.13},
  url = {https://doi.org/10.11648/j.ajz.20180102.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajz.20180102.13},
  abstract = {Staphylococcus aureus Food Poisoning is a common cause of food-borne disease worldwide. Of particular relevance is the ability of some Staphylococcus aureus strains to produce heat stable enterotoxins that cause staphylococcal food poisoning, which ranks as one of the most prevalent worldwide causes of gastroenteritis. Several studies have shown that 15% to 80% of the Staphylococcus aureus isolated from various sources (dairy products, ice heavy cream, meat products) is able to produce enterotoxin. Classically, enterotoxins from Staphylococcus aureus strains can be classified into 18 serological types: A-U (except S, F and T), most of these serotypes are heat stable. The percentage of samples of milk and milk products contaminated with S. aurues in the current study were 46% which is higher than that reported by who detected S. aureus in 17 % and 32 % respectively of the analyzed samples of milk and milk products. Staphylococcal enterotoxins are low molecular weight proteins (MW 26.900 – 29,600).They are encoded by genes embedded in mobile genetic elements such as phages and pathogenicity islands .There are several methods for detection of enterotoxigeneic bacteria. The phonotypical methods are not reliable in specificity, because staphylococcal enterotoxins serotypes are antigenically similar. On the other hand, commercial serological kits can not detect all the serotypes and are limited in serotypes (A-B). Therefore, molecular techniques such as multiplex polymerase chain reaction and real-time polymerase chain reaction are recommended for detection of Staphylococcus aureus enterotoxins genes.},
 year = {2019}
}

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TY  - JOUR
T1  - Prevalence of Staphylococcus aureus in Raw Milk and Some Dairy Products in Port Said Governorate
AU  - Saly Mamoun Elsayed Toubar
AU  - Abdllah Abdelkader Elbialy
AU  - Mahmoud Mohamed Mohamed Zaky
AU  - Ahmed Samy El-Shafey
Y1  - 2019/01/25
PY  - 2019
N1  - https://doi.org/10.11648/j.ajz.20180102.13
DO  - 10.11648/j.ajz.20180102.13
T2  - American Journal of Zoology
JF  - American Journal of Zoology
JO  - American Journal of Zoology
SP  - 40
EP  - 46
PB  - Science Publishing Group
SN  - 2994-7413
UR  - https://doi.org/10.11648/j.ajz.20180102.13
AB  - Staphylococcus aureus Food Poisoning is a common cause of food-borne disease worldwide. Of particular relevance is the ability of some Staphylococcus aureus strains to produce heat stable enterotoxins that cause staphylococcal food poisoning, which ranks as one of the most prevalent worldwide causes of gastroenteritis. Several studies have shown that 15% to 80% of the Staphylococcus aureus isolated from various sources (dairy products, ice heavy cream, meat products) is able to produce enterotoxin. Classically, enterotoxins from Staphylococcus aureus strains can be classified into 18 serological types: A-U (except S, F and T), most of these serotypes are heat stable. The percentage of samples of milk and milk products contaminated with S. aurues in the current study were 46% which is higher than that reported by who detected S. aureus in 17 % and 32 % respectively of the analyzed samples of milk and milk products. Staphylococcal enterotoxins are low molecular weight proteins (MW 26.900 – 29,600).They are encoded by genes embedded in mobile genetic elements such as phages and pathogenicity islands .There are several methods for detection of enterotoxigeneic bacteria. The phonotypical methods are not reliable in specificity, because staphylococcal enterotoxins serotypes are antigenically similar. On the other hand, commercial serological kits can not detect all the serotypes and are limited in serotypes (A-B). Therefore, molecular techniques such as multiplex polymerase chain reaction and real-time polymerase chain reaction are recommended for detection of Staphylococcus aureus enterotoxins genes.
VL  - 1
IS  - 2
ER  -

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Author Information

Saly Mamoun Elsayed Toubar

Botany Department, Faculty of Science, Port-Said University, Port-Said, Egypt
Abdllah Abdelkader Elbialy

Microbiology and Immunology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt
Mahmoud Mohamed Mohamed Zaky

Botany Department, Faculty of Science, Port-Said University, Port-Said, Egypt
Ahmed Samy El-Shafey

Microbiology Section, Faculty of Science, Tanta University, Tanta, Egypt

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APA Style

Saly Mamoun Elsayed Toubar, Abdllah Abdelkader Elbialy, Mahmoud Mohamed Mohamed Zaky, Ahmed Samy El-Shafey. (2019). Prevalence of Staphylococcus aureus in Raw Milk and Some Dairy Products in Port Said Governorate. American Journal of Zoology, 1(2), 40-46. https://doi.org/10.11648/j.ajz.20180102.13

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ACS Style

Saly Mamoun Elsayed Toubar; Abdllah Abdelkader Elbialy; Mahmoud Mohamed Mohamed Zaky; Ahmed Samy El-Shafey. Prevalence of Staphylococcus aureus in Raw Milk and Some Dairy Products in Port Said Governorate. Am. J. Zool. 2019, 1(2), 40-46. doi: 10.11648/j.ajz.20180102.13

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AMA Style

Saly Mamoun Elsayed Toubar, Abdllah Abdelkader Elbialy, Mahmoud Mohamed Mohamed Zaky, Ahmed Samy El-Shafey. Prevalence of Staphylococcus aureus in Raw Milk and Some Dairy Products in Port Said Governorate. Am J Zool. 2019;1(2):40-46. doi: 10.11648/j.ajz.20180102.13

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@article{10.11648/j.ajz.20180102.13,
  author = {Saly Mamoun Elsayed Toubar and Abdllah Abdelkader Elbialy and Mahmoud Mohamed Mohamed Zaky and Ahmed Samy El-Shafey},
  title = {Prevalence of Staphylococcus aureus in Raw Milk and Some Dairy Products in Port Said Governorate},
  journal = {American Journal of Zoology},
  volume = {1},
  number = {2},
  pages = {40-46},
  doi = {10.11648/j.ajz.20180102.13},
  url = {https://doi.org/10.11648/j.ajz.20180102.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajz.20180102.13},
  abstract = {Staphylococcus aureus Food Poisoning is a common cause of food-borne disease worldwide. Of particular relevance is the ability of some Staphylococcus aureus strains to produce heat stable enterotoxins that cause staphylococcal food poisoning, which ranks as one of the most prevalent worldwide causes of gastroenteritis. Several studies have shown that 15% to 80% of the Staphylococcus aureus isolated from various sources (dairy products, ice heavy cream, meat products) is able to produce enterotoxin. Classically, enterotoxins from Staphylococcus aureus strains can be classified into 18 serological types: A-U (except S, F and T), most of these serotypes are heat stable. The percentage of samples of milk and milk products contaminated with S. aurues in the current study were 46% which is higher than that reported by who detected S. aureus in 17 % and 32 % respectively of the analyzed samples of milk and milk products. Staphylococcal enterotoxins are low molecular weight proteins (MW 26.900 – 29,600).They are encoded by genes embedded in mobile genetic elements such as phages and pathogenicity islands .There are several methods for detection of enterotoxigeneic bacteria. The phonotypical methods are not reliable in specificity, because staphylococcal enterotoxins serotypes are antigenically similar. On the other hand, commercial serological kits can not detect all the serotypes and are limited in serotypes (A-B). Therefore, molecular techniques such as multiplex polymerase chain reaction and real-time polymerase chain reaction are recommended for detection of Staphylococcus aureus enterotoxins genes.},
 year = {2019}
}

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TY  - JOUR
T1  - Prevalence of Staphylococcus aureus in Raw Milk and Some Dairy Products in Port Said Governorate
AU  - Saly Mamoun Elsayed Toubar
AU  - Abdllah Abdelkader Elbialy
AU  - Mahmoud Mohamed Mohamed Zaky
AU  - Ahmed Samy El-Shafey
Y1  - 2019/01/25
PY  - 2019
N1  - https://doi.org/10.11648/j.ajz.20180102.13
DO  - 10.11648/j.ajz.20180102.13
T2  - American Journal of Zoology
JF  - American Journal of Zoology
JO  - American Journal of Zoology
SP  - 40
EP  - 46
PB  - Science Publishing Group
SN  - 2994-7413
UR  - https://doi.org/10.11648/j.ajz.20180102.13
AB  - Staphylococcus aureus Food Poisoning is a common cause of food-borne disease worldwide. Of particular relevance is the ability of some Staphylococcus aureus strains to produce heat stable enterotoxins that cause staphylococcal food poisoning, which ranks as one of the most prevalent worldwide causes of gastroenteritis. Several studies have shown that 15% to 80% of the Staphylococcus aureus isolated from various sources (dairy products, ice heavy cream, meat products) is able to produce enterotoxin. Classically, enterotoxins from Staphylococcus aureus strains can be classified into 18 serological types: A-U (except S, F and T), most of these serotypes are heat stable. The percentage of samples of milk and milk products contaminated with S. aurues in the current study were 46% which is higher than that reported by who detected S. aureus in 17 % and 32 % respectively of the analyzed samples of milk and milk products. Staphylococcal enterotoxins are low molecular weight proteins (MW 26.900 – 29,600).They are encoded by genes embedded in mobile genetic elements such as phages and pathogenicity islands .There are several methods for detection of enterotoxigeneic bacteria. The phonotypical methods are not reliable in specificity, because staphylococcal enterotoxins serotypes are antigenically similar. On the other hand, commercial serological kits can not detect all the serotypes and are limited in serotypes (A-B). Therefore, molecular techniques such as multiplex polymerase chain reaction and real-time polymerase chain reaction are recommended for detection of Staphylococcus aureus enterotoxins genes.
VL  - 1
IS  - 2
ER  -

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