Anticoagulant Activity of Rhamnan Sulfate Isolated from Commercially Cultured <i>Monostroma nitidum</i>

Youichi Yamashiro; Masahiro Nakamura; Takuya Yogi; Takeshi Teruya; Teruko Konishi; Shuntoku Uechi; Masakuni Tako

doi:doi:10.11648/j.ijbmr.20170503.12

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Anticoagulant Activity of Rhamnan Sulfate Isolated from Commercially Cultured Monostroma nitidum

Youichi Yamashiro, Masahiro Nakamura, Takuya Yogi, Takeshi Teruya, Teruko Konishi, Shuntoku Uechi, Masakuni Tako

Published in International Journal of Biomedical Materials Research (Volume 5, Issue 3)

Received: 11 April 2017 Accepted: 2 May 2017 Published: 21 June 2017

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Abstract

The green seaweed, Monostroma nitidum, is widespread in Japan. In Okinawa Prefecture, the production of seaweed is performed using culture-nets that are seeded artificially. Algae contain a soluble polysaccharide, rhamnan sulfate. To estimate its applicability, the anticoagulant activity of rhamnan sulfate was investigated. Rhamanan sulfate was fractionated by ion-exchange chromatography on a DEAE-sepharose column, and two fractions (A and B) were obtained. Partially hydrolyzed rhamnan sulfates with different molecular mass (C1, C2 and C3) were also prepared. The activated partial thromboplastin time (APTT) test, prothrombin time (PT) and thrombin time (TT) were applied using human plasma and compared with standard heparin (174 units/mg). The native rhamnan sulfate (molecular mass, 630 kDa; sulfuric acid content, 22.7%), fraction A (12.4%) and fraction B (27.8%) showed approximately 73% APTT activity in comparison with that of standard heparin, but fraction C2 (molecular mass, 450 kDa) had a higher activity than that of the standard (107%). On the other hand, in the PT assay, all fractions except fraction C2 and C3 (370 kDa) showed higher activity approximately 120-155% greater than that of standard heparin. The TT activity of rhamnan sulfate depended on the sulfate content, and that of fraction B, which has high sulfuric acid content (27.8%), was 135-173% greater than that of heparin. The sulfate groups of L-rhamnosyl residues and carboxyl group of D-glucuronosyl residue on the trisaccharide side chains of the rhamnan sulfate might interact strongly with the active site of thrombin molecules. The results and discussion suggested that rhamnan sulfate from commercially cultured Monostroma nitidum could be a potential anticoagulant polysaccharide.

Published in	International Journal of Biomedical Materials Research (Volume 5, Issue 3)
DOI	10.11648/j.ijbmr.20170503.12
Page(s)	37-43
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Monostroma nitidum, Commercially Cultured, Seaweed Polysaccharide, Rhamnan Sulfate, Anticoagulant Activity

References

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Youichi Yamashiro, Masahiro Nakamura, Takuya Yogi, Takeshi Teruya, Teruko Konishi, et al. (2017). Anticoagulant Activity of Rhamnan Sulfate Isolated from Commercially Cultured Monostroma nitidum. International Journal of Biomedical Materials Research, 5(3), 37-43. https://doi.org/10.11648/j.ijbmr.20170503.12

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ACS Style

Youichi Yamashiro; Masahiro Nakamura; Takuya Yogi; Takeshi Teruya; Teruko Konishi, et al. Anticoagulant Activity of Rhamnan Sulfate Isolated from Commercially Cultured Monostroma nitidum. Int. J. Biomed. Mater. Res. 2017, 5(3), 37-43. doi: 10.11648/j.ijbmr.20170503.12

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AMA Style

Youichi Yamashiro, Masahiro Nakamura, Takuya Yogi, Takeshi Teruya, Teruko Konishi, et al. Anticoagulant Activity of Rhamnan Sulfate Isolated from Commercially Cultured Monostroma nitidum. Int J Biomed Mater Res. 2017;5(3):37-43. doi: 10.11648/j.ijbmr.20170503.12

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@article{10.11648/j.ijbmr.20170503.12,
  author = {Youichi Yamashiro and Masahiro Nakamura and Takuya Yogi and Takeshi Teruya and Teruko Konishi and Shuntoku Uechi and Masakuni Tako},
  title = {Anticoagulant Activity of Rhamnan Sulfate Isolated from Commercially Cultured Monostroma nitidum},
  journal = {International Journal of Biomedical Materials Research},
  volume = {5},
  number = {3},
  pages = {37-43},
  doi = {10.11648/j.ijbmr.20170503.12},
  url = {https://doi.org/10.11648/j.ijbmr.20170503.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbmr.20170503.12},
  abstract = {The green seaweed, Monostroma nitidum, is widespread in Japan. In Okinawa Prefecture, the production of seaweed is performed using culture-nets that are seeded artificially. Algae contain a soluble polysaccharide, rhamnan sulfate. To estimate its applicability, the anticoagulant activity of rhamnan sulfate was investigated. Rhamanan sulfate was fractionated by ion-exchange chromatography on a DEAE-sepharose column, and two fractions (A and B) were obtained. Partially hydrolyzed rhamnan sulfates with different molecular mass (C1, C2 and C3) were also prepared. The activated partial thromboplastin time (APTT) test, prothrombin time (PT) and thrombin time (TT) were applied using human plasma and compared with standard heparin (174 units/mg). The native rhamnan sulfate (molecular mass, 630 kDa; sulfuric acid content, 22.7%), fraction A (12.4%) and fraction B (27.8%) showed approximately 73% APTT activity in comparison with that of standard heparin, but fraction C2 (molecular mass, 450 kDa) had a higher activity than that of the standard (107%). On the other hand, in the PT assay, all fractions except fraction C2 and C3 (370 kDa) showed higher activity approximately 120-155% greater than that of standard heparin. The TT activity of rhamnan sulfate depended on the sulfate content, and that of fraction B, which has high sulfuric acid content (27.8%), was 135-173% greater than that of heparin. The sulfate groups of L-rhamnosyl residues and carboxyl group of D-glucuronosyl residue on the trisaccharide side chains of the rhamnan sulfate might interact strongly with the active site of thrombin molecules. The results and discussion suggested that rhamnan sulfate from commercially cultured Monostroma nitidum could be a potential anticoagulant polysaccharide.},
 year = {2017}
}

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TY  - JOUR
T1  - Anticoagulant Activity of Rhamnan Sulfate Isolated from Commercially Cultured Monostroma nitidum
AU  - Youichi Yamashiro
AU  - Masahiro Nakamura
AU  - Takuya Yogi
AU  - Takeshi Teruya
AU  - Teruko Konishi
AU  - Shuntoku Uechi
AU  - Masakuni Tako
Y1  - 2017/06/21
PY  - 2017
N1  - https://doi.org/10.11648/j.ijbmr.20170503.12
DO  - 10.11648/j.ijbmr.20170503.12
T2  - International Journal of Biomedical Materials Research
JF  - International Journal of Biomedical Materials Research
JO  - International Journal of Biomedical Materials Research
SP  - 37
EP  - 43
PB  - Science Publishing Group
SN  - 2330-7579
UR  - https://doi.org/10.11648/j.ijbmr.20170503.12
AB  - The green seaweed, Monostroma nitidum, is widespread in Japan. In Okinawa Prefecture, the production of seaweed is performed using culture-nets that are seeded artificially. Algae contain a soluble polysaccharide, rhamnan sulfate. To estimate its applicability, the anticoagulant activity of rhamnan sulfate was investigated. Rhamanan sulfate was fractionated by ion-exchange chromatography on a DEAE-sepharose column, and two fractions (A and B) were obtained. Partially hydrolyzed rhamnan sulfates with different molecular mass (C1, C2 and C3) were also prepared. The activated partial thromboplastin time (APTT) test, prothrombin time (PT) and thrombin time (TT) were applied using human plasma and compared with standard heparin (174 units/mg). The native rhamnan sulfate (molecular mass, 630 kDa; sulfuric acid content, 22.7%), fraction A (12.4%) and fraction B (27.8%) showed approximately 73% APTT activity in comparison with that of standard heparin, but fraction C2 (molecular mass, 450 kDa) had a higher activity than that of the standard (107%). On the other hand, in the PT assay, all fractions except fraction C2 and C3 (370 kDa) showed higher activity approximately 120-155% greater than that of standard heparin. The TT activity of rhamnan sulfate depended on the sulfate content, and that of fraction B, which has high sulfuric acid content (27.8%), was 135-173% greater than that of heparin. The sulfate groups of L-rhamnosyl residues and carboxyl group of D-glucuronosyl residue on the trisaccharide side chains of the rhamnan sulfate might interact strongly with the active site of thrombin molecules. The results and discussion suggested that rhamnan sulfate from commercially cultured Monostroma nitidum could be a potential anticoagulant polysaccharide.
VL  - 5
IS  - 3
ER  -

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Author Information

Youichi Yamashiro

Department of Subtropical Bioscience and Biotechnology, Faculty of Agriculture, University of the Ryukyus, Nishihara, Okinawa, Japan
Masahiro Nakamura

Applied Life Science, United Graduate School of Agricultural Sciences, Kagoshima University, Kagoshima, Kagoshima, Japan
Takuya Yogi

Department of Subtropical Bioscience and Biotechnology, Faculty of Agriculture, University of the Ryukyus, Nishihara, Okinawa, Japan
Takeshi Teruya

Department of Subtropical Bioscience and Biotechnology, Faculty of Agriculture, University of the Ryukyus, Nishihara, Okinawa, Japan
Teruko Konishi

Department of Subtropical Bioscience and Biotechnology, Faculty of Agriculture, University of the Ryukyus, Nishihara, Okinawa, Japan
Shuntoku Uechi

Department of Subtropical Bioscience and Biotechnology, Faculty of Agriculture, University of the Ryukyus, Nishihara, Okinawa, Japan
Masakuni Tako

Department of Subtropical Bioscience and Biotechnology, Faculty of Agriculture, University of the Ryukyus, Nishihara, Okinawa, Japan

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APA Style

Youichi Yamashiro, Masahiro Nakamura, Takuya Yogi, Takeshi Teruya, Teruko Konishi, et al. (2017). Anticoagulant Activity of Rhamnan Sulfate Isolated from Commercially Cultured Monostroma nitidum. International Journal of Biomedical Materials Research, 5(3), 37-43. https://doi.org/10.11648/j.ijbmr.20170503.12

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ACS Style

Youichi Yamashiro; Masahiro Nakamura; Takuya Yogi; Takeshi Teruya; Teruko Konishi, et al. Anticoagulant Activity of Rhamnan Sulfate Isolated from Commercially Cultured Monostroma nitidum. Int. J. Biomed. Mater. Res. 2017, 5(3), 37-43. doi: 10.11648/j.ijbmr.20170503.12

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AMA Style

Youichi Yamashiro, Masahiro Nakamura, Takuya Yogi, Takeshi Teruya, Teruko Konishi, et al. Anticoagulant Activity of Rhamnan Sulfate Isolated from Commercially Cultured Monostroma nitidum. Int J Biomed Mater Res. 2017;5(3):37-43. doi: 10.11648/j.ijbmr.20170503.12

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@article{10.11648/j.ijbmr.20170503.12,
  author = {Youichi Yamashiro and Masahiro Nakamura and Takuya Yogi and Takeshi Teruya and Teruko Konishi and Shuntoku Uechi and Masakuni Tako},
  title = {Anticoagulant Activity of Rhamnan Sulfate Isolated from Commercially Cultured Monostroma nitidum},
  journal = {International Journal of Biomedical Materials Research},
  volume = {5},
  number = {3},
  pages = {37-43},
  doi = {10.11648/j.ijbmr.20170503.12},
  url = {https://doi.org/10.11648/j.ijbmr.20170503.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbmr.20170503.12},
  abstract = {The green seaweed, Monostroma nitidum, is widespread in Japan. In Okinawa Prefecture, the production of seaweed is performed using culture-nets that are seeded artificially. Algae contain a soluble polysaccharide, rhamnan sulfate. To estimate its applicability, the anticoagulant activity of rhamnan sulfate was investigated. Rhamanan sulfate was fractionated by ion-exchange chromatography on a DEAE-sepharose column, and two fractions (A and B) were obtained. Partially hydrolyzed rhamnan sulfates with different molecular mass (C1, C2 and C3) were also prepared. The activated partial thromboplastin time (APTT) test, prothrombin time (PT) and thrombin time (TT) were applied using human plasma and compared with standard heparin (174 units/mg). The native rhamnan sulfate (molecular mass, 630 kDa; sulfuric acid content, 22.7%), fraction A (12.4%) and fraction B (27.8%) showed approximately 73% APTT activity in comparison with that of standard heparin, but fraction C2 (molecular mass, 450 kDa) had a higher activity than that of the standard (107%). On the other hand, in the PT assay, all fractions except fraction C2 and C3 (370 kDa) showed higher activity approximately 120-155% greater than that of standard heparin. The TT activity of rhamnan sulfate depended on the sulfate content, and that of fraction B, which has high sulfuric acid content (27.8%), was 135-173% greater than that of heparin. The sulfate groups of L-rhamnosyl residues and carboxyl group of D-glucuronosyl residue on the trisaccharide side chains of the rhamnan sulfate might interact strongly with the active site of thrombin molecules. The results and discussion suggested that rhamnan sulfate from commercially cultured Monostroma nitidum could be a potential anticoagulant polysaccharide.},
 year = {2017}
}

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TY  - JOUR
T1  - Anticoagulant Activity of Rhamnan Sulfate Isolated from Commercially Cultured Monostroma nitidum
AU  - Youichi Yamashiro
AU  - Masahiro Nakamura
AU  - Takuya Yogi
AU  - Takeshi Teruya
AU  - Teruko Konishi
AU  - Shuntoku Uechi
AU  - Masakuni Tako
Y1  - 2017/06/21
PY  - 2017
N1  - https://doi.org/10.11648/j.ijbmr.20170503.12
DO  - 10.11648/j.ijbmr.20170503.12
T2  - International Journal of Biomedical Materials Research
JF  - International Journal of Biomedical Materials Research
JO  - International Journal of Biomedical Materials Research
SP  - 37
EP  - 43
PB  - Science Publishing Group
SN  - 2330-7579
UR  - https://doi.org/10.11648/j.ijbmr.20170503.12
AB  - The green seaweed, Monostroma nitidum, is widespread in Japan. In Okinawa Prefecture, the production of seaweed is performed using culture-nets that are seeded artificially. Algae contain a soluble polysaccharide, rhamnan sulfate. To estimate its applicability, the anticoagulant activity of rhamnan sulfate was investigated. Rhamanan sulfate was fractionated by ion-exchange chromatography on a DEAE-sepharose column, and two fractions (A and B) were obtained. Partially hydrolyzed rhamnan sulfates with different molecular mass (C1, C2 and C3) were also prepared. The activated partial thromboplastin time (APTT) test, prothrombin time (PT) and thrombin time (TT) were applied using human plasma and compared with standard heparin (174 units/mg). The native rhamnan sulfate (molecular mass, 630 kDa; sulfuric acid content, 22.7%), fraction A (12.4%) and fraction B (27.8%) showed approximately 73% APTT activity in comparison with that of standard heparin, but fraction C2 (molecular mass, 450 kDa) had a higher activity than that of the standard (107%). On the other hand, in the PT assay, all fractions except fraction C2 and C3 (370 kDa) showed higher activity approximately 120-155% greater than that of standard heparin. The TT activity of rhamnan sulfate depended on the sulfate content, and that of fraction B, which has high sulfuric acid content (27.8%), was 135-173% greater than that of heparin. The sulfate groups of L-rhamnosyl residues and carboxyl group of D-glucuronosyl residue on the trisaccharide side chains of the rhamnan sulfate might interact strongly with the active site of thrombin molecules. The results and discussion suggested that rhamnan sulfate from commercially cultured Monostroma nitidum could be a potential anticoagulant polysaccharide.
VL  - 5
IS  - 3
ER  -

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