The Effect of Paclitaxel on the Viability of U14 Cells

Gao Xuesong; Lin Shaoqiang; Wang Xiaoyu

doi:doi:10.11648/j.jgo.20200803.11

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The Effect of Paclitaxel on the Viability of U14 Cells

Gao Xuesong, Lin Shaoqiang, Wang Xiaoyu

Published in Journal of Gynecology and Obstetrics (Volume 8, Issue 3)

Received: 18 April 2020 Accepted: 29 April 2020 Published: 11 May 2020

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Abstract

The research is aimed to study the effect of paclitaxel on the viability of U14 cell line of cervical cancer, and to provide new ideas for further exploring the mechanism of paclitaxel chemotherapy to cervical cancer. Then, different concentrations of paclitaxel were used to treat U14 cells of cervical cancer in logarithmic phase growth. After culturing these cells for 24h, 48h or 72h, MTT method and automatic enzyme-linked immunosorbent assay system were used to evaluate the survival rate of cultured cells with different concentrations of paclitaxel. These results showed that paclitaxel has a significant inhibitory effect on the proliferation of U14 cells in a concentration- and time-dependent manner, compared with the control group. Paclitaxel concentration of the IC50 of U14 cells was 168.8μg/ml at 24h, 22. 15 μg/ml at 48h and 8. 04μg/ml at 72 h. As the time increased, the IC50 value of U14 cells gradually decreased. The results of linear regression analysis are as follows: 24 h, y=-0.005x + 1.344, R²=0.779; 48 h, y=-0.013x +0.788, R²=0.923; 72 h, y=-0.056x + 0.950, R²=0.908. The dose-effect curve of paclitaxel on U14 cells for 48 h and the trend of linear regression fitting are better, and the growth inhibition of cells shows a clear relationship between time and dose. In conclusion, paclitaxel has an obvious inhibitory effect on the activity of cervical cancer U14 cells in mice, which provides new ideas for further exploring the mechanism of cervical cancer paclitaxel chemotherapy.

Published in	Journal of Gynecology and Obstetrics (Volume 8, Issue 3)
DOI	10.11648/j.jgo.20200803.11
Page(s)	51-54
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2020. Published by Science Publishing Group

Keywords

Paclitaxel, Cervical Cancer, Cell Viability, U14 Cell

References

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[8]	Wang H, Zhu Y, Hao C, Fan J, Liu Y, Wang Y. Establishment of a drug-resistant human cervical cancer cell line and research on its drug-resistance. Journal of cancer research and therapeutics 2019, 15 (6): 1221-1225.
[9]	Lee BE, Choi BY, Hong DK, Kim JH, Lee SH, Kho AR, Kim H, Choi HC, Suh SW. The cancer chemotherapeutic agent paclitaxel (Paclitaxel) reduces hippocampal neurogenesis via down-regulation of vesicular zinc. Scientific reports 2017, 7 (1): 11667.
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APA Style

Gao Xuesong, Lin Shaoqiang, Wang Xiaoyu. (2020). The Effect of Paclitaxel on the Viability of U14 Cells. Journal of Gynecology and Obstetrics, 8(3), 51-54. https://doi.org/10.11648/j.jgo.20200803.11

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ACS Style

Gao Xuesong; Lin Shaoqiang; Wang Xiaoyu. The Effect of Paclitaxel on the Viability of U14 Cells. J. Gynecol. Obstet. 2020, 8(3), 51-54. doi: 10.11648/j.jgo.20200803.11

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AMA Style

Gao Xuesong, Lin Shaoqiang, Wang Xiaoyu. The Effect of Paclitaxel on the Viability of U14 Cells. J Gynecol Obstet. 2020;8(3):51-54. doi: 10.11648/j.jgo.20200803.11

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@article{10.11648/j.jgo.20200803.11,
  author = {Gao Xuesong and Lin Shaoqiang and Wang Xiaoyu},
  title = {The Effect of Paclitaxel on the Viability of U14 Cells},
  journal = {Journal of Gynecology and Obstetrics},
  volume = {8},
  number = {3},
  pages = {51-54},
  doi = {10.11648/j.jgo.20200803.11},
  url = {https://doi.org/10.11648/j.jgo.20200803.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jgo.20200803.11},
  abstract = {The research is aimed to study the effect of paclitaxel on the viability of U14 cell line of cervical cancer, and to provide new ideas for further exploring the mechanism of paclitaxel chemotherapy to cervical cancer. Then, different concentrations of paclitaxel were used to treat U14 cells of cervical cancer in logarithmic phase growth. After culturing these cells for 24h, 48h or 72h, MTT method and automatic enzyme-linked immunosorbent assay system were used to evaluate the survival rate of cultured cells with different concentrations of paclitaxel. These results showed that paclitaxel has a significant inhibitory effect on the proliferation of U14 cells in a concentration- and time-dependent manner, compared with the control group. Paclitaxel concentration of the IC50 of U14 cells was 168.8μg/ml at 24h, 22. 15 μg/ml at 48h and 8. 04μg/ml at 72 h. As the time increased, the IC50 value of U14 cells gradually decreased. The results of linear regression analysis are as follows: 24 h, y=-0.005x + 1.344, R2=0.779; 48 h, y=-0.013x +0.788, R2=0.923; 72 h, y=-0.056x + 0.950, R2=0.908. The dose-effect curve of paclitaxel on U14 cells for 48 h and the trend of linear regression fitting are better, and the growth inhibition of cells shows a clear relationship between time and dose. In conclusion, paclitaxel has an obvious inhibitory effect on the activity of cervical cancer U14 cells in mice, which provides new ideas for further exploring the mechanism of cervical cancer paclitaxel chemotherapy.},
 year = {2020}
}

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TY - JOUR
T1 - The Effect of Paclitaxel on the Viability of U14 Cells
AU - Gao Xuesong
AU - Lin Shaoqiang
AU - Wang Xiaoyu
Y1 - 2020/05/11
PY - 2020
N1 - https://doi.org/10.11648/j.jgo.20200803.11
DO - 10.11648/j.jgo.20200803.11
T2 - Journal of Gynecology and Obstetrics
JF - Journal of Gynecology and Obstetrics
JO - Journal of Gynecology and Obstetrics
SP - 51
EP - 54
PB - Science Publishing Group
SN - 2376-7820
UR - https://doi.org/10.11648/j.jgo.20200803.11
AB - The research is aimed to study the effect of paclitaxel on the viability of U14 cell line of cervical cancer, and to provide new ideas for further exploring the mechanism of paclitaxel chemotherapy to cervical cancer. Then, different concentrations of paclitaxel were used to treat U14 cells of cervical cancer in logarithmic phase growth. After culturing these cells for 24h, 48h or 72h, MTT method and automatic enzyme-linked immunosorbent assay system were used to evaluate the survival rate of cultured cells with different concentrations of paclitaxel. These results showed that paclitaxel has a significant inhibitory effect on the proliferation of U14 cells in a concentration- and time-dependent manner, compared with the control group. Paclitaxel concentration of the IC50 of U14 cells was 168.8μg/ml at 24h, 22. 15 μg/ml at 48h and 8. 04μg/ml at 72 h. As the time increased, the IC50 value of U14 cells gradually decreased. The results of linear regression analysis are as follows: 24 h, y=-0.005x + 1.344, R2=0.779; 48 h, y=-0.013x +0.788, R2=0.923; 72 h, y=-0.056x + 0.950, R2=0.908. The dose-effect curve of paclitaxel on U14 cells for 48 h and the trend of linear regression fitting are better, and the growth inhibition of cells shows a clear relationship between time and dose. In conclusion, paclitaxel has an obvious inhibitory effect on the activity of cervical cancer U14 cells in mice, which provides new ideas for further exploring the mechanism of cervical cancer paclitaxel chemotherapy.
VL - 8
IS - 3
ER -

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Author Information

Gao Xuesong

Department of Obstetrics and Gynecology, The First Affiliated Hospital of Jinan University, Guangzhou, China
Lin Shaoqiang

The Hospital Management Department of Guangdong Pharmaceutical University, Guangzhou, China
Wang Xiaoyu

Department of Obstetrics and Gynecology, The First Affiliated Hospital of Jinan University, Guangzhou, China

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APA Style

Gao Xuesong, Lin Shaoqiang, Wang Xiaoyu. (2020). The Effect of Paclitaxel on the Viability of U14 Cells. Journal of Gynecology and Obstetrics, 8(3), 51-54. https://doi.org/10.11648/j.jgo.20200803.11

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ACS Style

Gao Xuesong; Lin Shaoqiang; Wang Xiaoyu. The Effect of Paclitaxel on the Viability of U14 Cells. J. Gynecol. Obstet. 2020, 8(3), 51-54. doi: 10.11648/j.jgo.20200803.11

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AMA Style

Gao Xuesong, Lin Shaoqiang, Wang Xiaoyu. The Effect of Paclitaxel on the Viability of U14 Cells. J Gynecol Obstet. 2020;8(3):51-54. doi: 10.11648/j.jgo.20200803.11

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@article{10.11648/j.jgo.20200803.11,
  author = {Gao Xuesong and Lin Shaoqiang and Wang Xiaoyu},
  title = {The Effect of Paclitaxel on the Viability of U14 Cells},
  journal = {Journal of Gynecology and Obstetrics},
  volume = {8},
  number = {3},
  pages = {51-54},
  doi = {10.11648/j.jgo.20200803.11},
  url = {https://doi.org/10.11648/j.jgo.20200803.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jgo.20200803.11},
  abstract = {The research is aimed to study the effect of paclitaxel on the viability of U14 cell line of cervical cancer, and to provide new ideas for further exploring the mechanism of paclitaxel chemotherapy to cervical cancer. Then, different concentrations of paclitaxel were used to treat U14 cells of cervical cancer in logarithmic phase growth. After culturing these cells for 24h, 48h or 72h, MTT method and automatic enzyme-linked immunosorbent assay system were used to evaluate the survival rate of cultured cells with different concentrations of paclitaxel. These results showed that paclitaxel has a significant inhibitory effect on the proliferation of U14 cells in a concentration- and time-dependent manner, compared with the control group. Paclitaxel concentration of the IC50 of U14 cells was 168.8μg/ml at 24h, 22. 15 μg/ml at 48h and 8. 04μg/ml at 72 h. As the time increased, the IC50 value of U14 cells gradually decreased. The results of linear regression analysis are as follows: 24 h, y=-0.005x + 1.344, R2=0.779; 48 h, y=-0.013x +0.788, R2=0.923; 72 h, y=-0.056x + 0.950, R2=0.908. The dose-effect curve of paclitaxel on U14 cells for 48 h and the trend of linear regression fitting are better, and the growth inhibition of cells shows a clear relationship between time and dose. In conclusion, paclitaxel has an obvious inhibitory effect on the activity of cervical cancer U14 cells in mice, which provides new ideas for further exploring the mechanism of cervical cancer paclitaxel chemotherapy.},
 year = {2020}
}

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