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The Effect of Paclitaxel on the Viability of U14 Cells
Gao Xuesong,
Lin Shaoqiang,
Wang Xiaoyu
Issue:
Volume 8, Issue 3, May 2020
Pages:
51-54
Received:
18 April 2020
Accepted:
29 April 2020
Published:
11 May 2020
Abstract: The research is aimed to study the effect of paclitaxel on the viability of U14 cell line of cervical cancer, and to provide new ideas for further exploring the mechanism of paclitaxel chemotherapy to cervical cancer. Then, different concentrations of paclitaxel were used to treat U14 cells of cervical cancer in logarithmic phase growth. After culturing these cells for 24h, 48h or 72h, MTT method and automatic enzyme-linked immunosorbent assay system were used to evaluate the survival rate of cultured cells with different concentrations of paclitaxel. These results showed that paclitaxel has a significant inhibitory effect on the proliferation of U14 cells in a concentration- and time-dependent manner, compared with the control group. Paclitaxel concentration of the IC50 of U14 cells was 168.8μg/ml at 24h, 22. 15 μg/ml at 48h and 8. 04μg/ml at 72 h. As the time increased, the IC50 value of U14 cells gradually decreased. The results of linear regression analysis are as follows: 24 h, y=-0.005x + 1.344, R2=0.779; 48 h, y=-0.013x +0.788, R2=0.923; 72 h, y=-0.056x + 0.950, R2=0.908. The dose-effect curve of paclitaxel on U14 cells for 48 h and the trend of linear regression fitting are better, and the growth inhibition of cells shows a clear relationship between time and dose. In conclusion, paclitaxel has an obvious inhibitory effect on the activity of cervical cancer U14 cells in mice, which provides new ideas for further exploring the mechanism of cervical cancer paclitaxel chemotherapy.
Abstract: The research is aimed to study the effect of paclitaxel on the viability of U14 cell line of cervical cancer, and to provide new ideas for further exploring the mechanism of paclitaxel chemotherapy to cervical cancer. Then, different concentrations of paclitaxel were used to treat U14 cells of cervical cancer in logarithmic phase growth. After cult...
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Laparoscopic Sacrocolpopexy vs Transvaginal Mesh Repair for Advanced Pelvic Organ Prolapse: 1 Year Results of a Multicenter Randomized Study
Juan Chen,
Tao Xu,
Xiaowei Zhang,
Keqin Hua,
Mei Ji,
Luwen Wang,
Huicheng Xu,
Jinghe Lang,
Abraham Morse,
Lan Zhu
Issue:
Volume 8, Issue 3, May 2020
Pages:
55-61
Received:
14 March 2020
Accepted:
17 April 2020
Published:
12 May 2020
Abstract: Backgrounds: Laparoscopic sacrocolpopexy is considered the most durable operation for the repair of advanced uterovaginal prolapse. However, there is still disagreement about whether the efficacy and safety of vaginally implanted mesh to address advanced uterovaginal prolapse is comparable to sacrocolpopexy. Our goal was to evaluate the anatomical and subjective outcomes of laparoscopic sacrocolpopexy versus transvaginal mesh in a randomized trial in China. Methods: A multicenter randomized trial was carried out at 6 tertiary hospitals in China. Patients with symptomatic advanced prolapse (stages III-IV) were enrolled. Between January 2013 and June 2014, a total of 100 women were randomized. 40 laparoscopic sacrocolpopexy procedures and 42 transvaginal mesh procedures were performed. Patients were randomized to undergo either laparoscopic sacrocolpopexy or transvaginal mesh. Results: At 1 year, the anatomic success rate was 92.5% in the laparoscopic sacrocolpopexy arm, compared with 83.3% in the transvaginal mesh group (P=0.35). Laparoscopic sacrocolpopexy was associated with better apical support. The laparoscopic sacrocolpopexy group had a longer operative time. Mesh exposures occurred in 2.5% of laparoscopic repairs vs. 2.4% of transvaginal mesh repairs. Conclusion: In a randomized trial, 1-year objective cure rates were not statistically different. However the success rate was 9.2% higher for laparoscopic sacrocolpopexy. The two procedures had comparable mesh exposure rates, and other complications were rare in both groups. Trial registration: clinicaltrials.gov (NCT01762384). The date of registration was Jan 7th, 2013. URL was https://clinicaltrials.gov/ct2/show/NCT01762384?term=NCT01762384&draw=2&rank=1.
Abstract: Backgrounds: Laparoscopic sacrocolpopexy is considered the most durable operation for the repair of advanced uterovaginal prolapse. However, there is still disagreement about whether the efficacy and safety of vaginally implanted mesh to address advanced uterovaginal prolapse is comparable to sacrocolpopexy. Our goal was to evaluate the anatomical ...
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High Blood Pressure in the Birth Room: Epidemiology and Outcome of Pregnancies at the General Hospital of Loandjili (Pointe-Noire, Congo)
Eouani Levy Max Emery,
Buambo Gauthier Régis Jostin,
Mokoko Jules Cesar,
Itoua Clautaire,
Potokoue Mpia Sekangue Samantha Nuelly,
Kombo Boukaka Davy,
Iloki Léon Hervé
Issue:
Volume 8, Issue 3, May 2020
Pages:
62-66
Received:
10 April 2020
Accepted:
27 April 2020
Published:
12 May 2020
Abstract: Objective: According to the World Health Organization, blood pressure disorders of pregnancy constitute a real public health problem of worldwide scope. It is an important factor of gravity, provider of a high maternal and perinatal morbidity and mortality in our maternities. The aim of this work is to study the epidemiological profile of pregnant women with hypertension and the outcome of their pregnancy. Methods: Descriptive cross-sectional study, carried out from January 1 to December 31, 2019, in the birthing block of the Obstetrics Gynecology service of the Loandjili General Hospital in Pointe-Noire, having included exhaustively and consecutively, all of them having given birth high blood pressure from a theoretical or ultrasound term of at least 22 weeks of amenorrhea or a birth weight of at least 500 g according to the WHO. New-borns of hypertensive mothers were also considered. The variables studied were socio-demographic, reproductive, linked to monitoring of pregnancy, clinical, relating to childbirth and maternal and perinatal prognosis. Results: Eighty and fourteen hypertensive pregnancies were recorded among 1677 admitted to the birthing room, a frequency of 5.6%. They were of a median age of 33, predominantly employed (60%), multigest (69%) and multiparous (44%). They were the most referred (66%) and admitted for high blood pressure or its complications in more than three quarters of cases (78%). Delivery was premature in more than half of the cases (54%), either spontaneously or induced. In this context, caesarean section was the preferred delivery route (77% of cases), performed urgently (94.4%) for severe maternal morbidity. Indications for emergency caesarean section were dominated by severe preeclampsia (67%), retroplacental hematoma (14%) and eclampsia (11%). One case of maternal death has been noted. Stillbirth was noted in 5% of cases (n=5). Neonatal morbidity was represented by poor adaptation to ectopic life (n=24 or 27%), hypotrophy (n=3 or 14%), prematurity (n=19 or 20%), neonatal resuscitation (n=24 or 27%) and the transfer to neonatology (n=45 or 48%). Conclusion: The association of high blood pressure and pregnancy remains frequent in our maternities. Given its high morbidity and mortality, it poses a real challenge for the obstetrician as to the outcome of pregnancies. The obstetrical prognosis and the improvement of the maternal and new-born prognosis go through the promotion of quality prenatal contacts and prevention.
Abstract: Objective: According to the World Health Organization, blood pressure disorders of pregnancy constitute a real public health problem of worldwide scope. It is an important factor of gravity, provider of a high maternal and perinatal morbidity and mortality in our maternities. The aim of this work is to study the epidemiological profile of pregnant ...
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To Determine the Incidence and Risk Factors Associated with Placenta Previa in a Tertiary Care Hospital of Pakistan
Sadia Asghar,
Samra Asghar Cheema,
Nafeesa Naz
Issue:
Volume 8, Issue 3, May 2020
Pages:
67-70
Received:
9 March 2020
Accepted:
21 April 2020
Published:
28 May 2020
Abstract: Placenta previa is the condition of pregnancy in which placenta implants in lower uterine segment, partially or completely covering the internal os. This condition may associate with many risk factors. Because with placenta previa pregnancy becomes high risk and fetomaternal morbidities and mortalities are also increased. By knowing the risk factors which are the main causative factors for placenta previa fetomaternal outcome can be optimized The purpose of this study is to find the incidence of placenta previa, and also determine the risk factors for placenta previa. This is descriptive prospective study. This study was done in tertiary care hospital of Pakistan. In this study total deliveries were 5381, total patient with previa were found 325 (6.03%). The risk factors of placenta previa are age more than 35 years (28.92%), smoking (20.16%), multiparity (44%), uterine scar (60.30%), previous evacuation and curettage (24.30%). According to age distribution to mostly patients about 135 out of 325 were found between age group of 36-40 years with percentage of 41.23%. Distribution of patients accrding to gestational age mostly found between age 33-35 weeks with percentage of 54.76 percent amd according to gravidity mostly found between G5–G7 with percentage of 42.46 percent. Aim of the study is to find out the risk factors associated with placenta previa and also determine the incidence of placenta previa in tertiary care hospital of Pakistan.
Abstract: Placenta previa is the condition of pregnancy in which placenta implants in lower uterine segment, partially or completely covering the internal os. This condition may associate with many risk factors. Because with placenta previa pregnancy becomes high risk and fetomaternal morbidities and mortalities are also increased. By knowing the risk factor...
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