Study on Characteristic Band Analysis of Soybean Protein, Soy Milk, Milk, and Coconut Milk Using SDS-PAGE Electrophoresis Technology

Zhang Yushan; Li Biyi; Shen Zhihua; Liu Yonggang

doi:doi:10.11648/j.sd.20231106.11

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Study on Characteristic Band Analysis of Soybean Protein, Soy Milk, Milk, and Coconut Milk Using SDS-PAGE Electrophoresis Technology

Zhang Yushan^*, Li Biyi, Shen Zhihua, Liu Yonggang

Published in Science Discovery (Volume 11, Issue 6)

Received: 17 September 2023 Accepted: 31 October 2023 Published: 8 November 2023

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Abstract

In order to find out the characteristic bands of different kinds of proteins, this study took soybean protein, vitamin milk, milk and coconut milk as the research objects, and SDS-PAGE gel electrophoresis was used to identify the characteristic bands of the above proteins. The experimental results showed that the polyacrylamide band of soybean protein consisted of: b and c protein bands. The typical protein bands of milk protein were c and d protein bands. The typical protein bands of coconut milk protein were c, d, and e protein bands. The typical protein bands of Vitasoy protein showed b, c, and d three protein bands. The b-band was the characteristic band of soybean protein, with a molecular weight of about 35 kDa. The d-band was the characteristic band of milk protein, with a molecular weight of about 30 kDa. The e-band was the characteristic band of coconut milk protein, with a molecular weight of around 25 kDa. Based on these characteristic bands of proteins, the experimental results also suggested that Vitasoy and coconut milk contain a certain amount of milk. These results indicated that SDS-PAGE gel could be used to find the characteristic bands of different kinds of proteins. Based on these characteristic bands, molecular identification of different types of proteins can be carried out.

Published in	Science Discovery (Volume 11, Issue 6)
DOI	10.11648/j.sd.20231106.11
Page(s)	189-192
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2023. Published by Science Publishing Group

Keywords

SDS-PAGE Gel Electrophoresis, Milk, Soybean Protein, Coconut Milk, Protein Characteristic Bands

References

[1]	马冠生, 张建芬, 张曼. 植物蛋白和动物蛋白，吃哪个更好 [J]. 农村新技术, 2022, No. 505 (09): 66.
[2]	赵博, 赵元寿, 苏小红. 大豆蛋白的研究进展 [J]. 甘肃科技纵横, 2021, 50 (12): 19-21+24.
[3]	张猛. 富含牛奶蛋白的饮料对女性运动后能量摄入的影响 [J]. 基因组学与应用生物学, 2019, 38 (04): 1883-1891.
[4]	林塬, 吴毓炜, 王焱等. 椰浆中椰子蛋白的提取、分离和鉴定 [J]. 热带作物学报, 2021, 42 (04): 1106-1112.
[5]	陈春梅, 王确, 周国强等. 植物蛋白及其产品应用的研究进展 [J]. 现代食品, 2022, 28 (20): 53-62.
[6]	梁华梁, 李珂, 冯策. 中国豆奶市场竞争情况盘点 [J]. 乳品与人类, 2020, No. 110 (01): 26-35.
[7]	杨硕, 李诗瑶, 王鸣秋等. 市售椰子汁 (植物蛋白饮料) 中椰子、大豆、花生源性成分鉴定的分子生物学方法 [J]. 基因组学与应用生物学, 2018, 37 (10): 4511-4517.
[8]	庞婕, 胥小容, 孔庆岩, 等. 运用PCR定量分析植物蛋白饮料中杏仁源性成分的研究 [J]. 食品安全导刊, 2022, 95-98.
[9]	项爱丽, 段晓然, 梅汝蕃, 等. 基于PCR检测技术鉴别羊肉掺假的研究进展. 食品工业, 2022, 43 (06): 267-270.
[10]	赵俊鑫, 李亚辉, 祁翰蒙, 等. 基于多种检测技术分析乳制品成分和质量的研究 [J]. 中国乳制品, 2023, 51 (01): 41-46.
[11]	Yuan Lin, Yan Wang, Zherong Ji, et al. Isolation, Purification, and Identification of Coconut Protein through SDS-PAGE, HPLC, and MALDI-TOF/TOF-MS [J]. Food Analytical Methods, 2020, 13 (prepublish).
[12]	Lili Guo, Yajun Wu, Mingchang Liu,, et al. Determination of edible bird's nests by FTIR and SDS-PAGE coupled with multivariate analysis [J]. Food Control, 2017, 80.
[13]	Uysal Reyhan Selin, Acar-Soykut Esra, Boyaci Ismail Hakki. Determination of yolk: white ratio of egg using SDS-PAGE. [J]. Food science and biotechnology, 2020, 29 (2).
[14]	孙潇慧, 李新玲, 霍胜楠等. 十二烷基硫酸钠-聚丙烯酰胺凝胶电泳技术及国内外蜂蜜掺伪检测技术研究进展 [J]. 食品安全质量检测学报, 2019, 10 (16): 5288-5292.
[15]	刘利娟. SDS-PAGE法对乳及乳制品中主要蛋白的定性和定量分析 [J]. 食品界, 2022 (03), 97-99.
[16]	张小慧, 王世伟, 席啸虎, 等. SDS-PAGE凝胶电泳对驴皮胶及常见伪品胶中的蛋白分析研究. 中医药导报, 2023, 29 (02): 47-49.

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APA Style

Yushan, Z., Biyi, L., Zhihua, S., Yonggang, L. (2023). Study on Characteristic Band Analysis of Soybean Protein, Soy Milk, Milk, and Coconut Milk Using SDS-PAGE Electrophoresis Technology. Science Discovery, 11(6), 189-192. https://doi.org/10.11648/j.sd.20231106.11

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ACS Style

Yushan, Z.; Biyi, L.; Zhihua, S.; Yonggang, L. Study on Characteristic Band Analysis of Soybean Protein, Soy Milk, Milk, and Coconut Milk Using SDS-PAGE Electrophoresis Technology. Sci. Discov. 2023, 11(6), 189-192. doi: 10.11648/j.sd.20231106.11

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AMA Style

Yushan Z, Biyi L, Zhihua S, Yonggang L. Study on Characteristic Band Analysis of Soybean Protein, Soy Milk, Milk, and Coconut Milk Using SDS-PAGE Electrophoresis Technology. Sci Discov. 2023;11(6):189-192. doi: 10.11648/j.sd.20231106.11

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@article{10.11648/j.sd.20231106.11,
  author = {Zhang Yushan and Li Biyi and Shen Zhihua and Liu Yonggang},
  title = {Study on Characteristic Band Analysis of Soybean Protein, Soy Milk, Milk, and Coconut Milk Using SDS-PAGE Electrophoresis Technology},
  journal = {Science Discovery},
  volume = {11},
  number = {6},
  pages = {189-192},
  doi = {10.11648/j.sd.20231106.11},
  url = {https://doi.org/10.11648/j.sd.20231106.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sd.20231106.11},
  abstract = {In order to find out the characteristic bands of different kinds of proteins, this study took soybean protein, vitamin milk, milk and coconut milk as the research objects, and SDS-PAGE gel electrophoresis was used to identify the characteristic bands of the above proteins. The experimental results showed that the polyacrylamide band of soybean protein consisted of: b and c protein bands. The typical protein bands of milk protein were c and d protein bands. The typical protein bands of coconut milk protein were c, d, and e protein bands. The typical protein bands of Vitasoy protein showed b, c, and d three protein bands. The b-band was the characteristic band of soybean protein, with a molecular weight of about 35 kDa. The d-band was the characteristic band of milk protein, with a molecular weight of about 30 kDa. The e-band was the characteristic band of coconut milk protein, with a molecular weight of around 25 kDa. Based on these characteristic bands of proteins, the experimental results also suggested that Vitasoy and coconut milk contain a certain amount of milk. These results indicated that SDS-PAGE gel could be used to find the characteristic bands of different kinds of proteins. Based on these characteristic bands, molecular identification of different types of proteins can be carried out.
},
 year = {2023}
}

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TY - JOUR
T1 - Study on Characteristic Band Analysis of Soybean Protein, Soy Milk, Milk, and Coconut Milk Using SDS-PAGE Electrophoresis Technology
AU - Zhang Yushan
AU - Li Biyi
AU - Shen Zhihua
AU - Liu Yonggang
Y1 - 2023/11/08
PY - 2023
N1 - https://doi.org/10.11648/j.sd.20231106.11
DO - 10.11648/j.sd.20231106.11
T2 - Science Discovery
JF - Science Discovery
JO - Science Discovery
SP - 189
EP - 192
PB - Science Publishing Group
SN - 2331-0650
UR - https://doi.org/10.11648/j.sd.20231106.11
AB - In order to find out the characteristic bands of different kinds of proteins, this study took soybean protein, vitamin milk, milk and coconut milk as the research objects, and SDS-PAGE gel electrophoresis was used to identify the characteristic bands of the above proteins. The experimental results showed that the polyacrylamide band of soybean protein consisted of: b and c protein bands. The typical protein bands of milk protein were c and d protein bands. The typical protein bands of coconut milk protein were c, d, and e protein bands. The typical protein bands of Vitasoy protein showed b, c, and d three protein bands. The b-band was the characteristic band of soybean protein, with a molecular weight of about 35 kDa. The d-band was the characteristic band of milk protein, with a molecular weight of about 30 kDa. The e-band was the characteristic band of coconut milk protein, with a molecular weight of around 25 kDa. Based on these characteristic bands of proteins, the experimental results also suggested that Vitasoy and coconut milk contain a certain amount of milk. These results indicated that SDS-PAGE gel could be used to find the characteristic bands of different kinds of proteins. Based on these characteristic bands, molecular identification of different types of proteins can be carried out.

VL - 11
IS - 6
ER -

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Author Information

Zhang Yushan

College of Materials and Food, University of Electronic Science and Technology of China, Zhongshan Institute, Zhongshan, China

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Li Biyi

College of Materials and Food, University of Electronic Science and Technology of China, Zhongshan Institute, Zhongshan, China

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Shen Zhihua

College of Materials and Food, University of Electronic Science and Technology of China, Zhongshan Institute, Zhongshan, China

Contact Email
Liu Yonggang

College of Materials and Food, University of Electronic Science and Technology of China, Zhongshan Institute, Zhongshan, China

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APA Style

Yushan, Z., Biyi, L., Zhihua, S., Yonggang, L. (2023). Study on Characteristic Band Analysis of Soybean Protein, Soy Milk, Milk, and Coconut Milk Using SDS-PAGE Electrophoresis Technology. Science Discovery, 11(6), 189-192. https://doi.org/10.11648/j.sd.20231106.11

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ACS Style

Yushan, Z.; Biyi, L.; Zhihua, S.; Yonggang, L. Study on Characteristic Band Analysis of Soybean Protein, Soy Milk, Milk, and Coconut Milk Using SDS-PAGE Electrophoresis Technology. Sci. Discov. 2023, 11(6), 189-192. doi: 10.11648/j.sd.20231106.11

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AMA Style

Yushan Z, Biyi L, Zhihua S, Yonggang L. Study on Characteristic Band Analysis of Soybean Protein, Soy Milk, Milk, and Coconut Milk Using SDS-PAGE Electrophoresis Technology. Sci Discov. 2023;11(6):189-192. doi: 10.11648/j.sd.20231106.11

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@article{10.11648/j.sd.20231106.11,
  author = {Zhang Yushan and Li Biyi and Shen Zhihua and Liu Yonggang},
  title = {Study on Characteristic Band Analysis of Soybean Protein, Soy Milk, Milk, and Coconut Milk Using SDS-PAGE Electrophoresis Technology},
  journal = {Science Discovery},
  volume = {11},
  number = {6},
  pages = {189-192},
  doi = {10.11648/j.sd.20231106.11},
  url = {https://doi.org/10.11648/j.sd.20231106.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sd.20231106.11},
  abstract = {In order to find out the characteristic bands of different kinds of proteins, this study took soybean protein, vitamin milk, milk and coconut milk as the research objects, and SDS-PAGE gel electrophoresis was used to identify the characteristic bands of the above proteins. The experimental results showed that the polyacrylamide band of soybean protein consisted of: b and c protein bands. The typical protein bands of milk protein were c and d protein bands. The typical protein bands of coconut milk protein were c, d, and e protein bands. The typical protein bands of Vitasoy protein showed b, c, and d three protein bands. The b-band was the characteristic band of soybean protein, with a molecular weight of about 35 kDa. The d-band was the characteristic band of milk protein, with a molecular weight of about 30 kDa. The e-band was the characteristic band of coconut milk protein, with a molecular weight of around 25 kDa. Based on these characteristic bands of proteins, the experimental results also suggested that Vitasoy and coconut milk contain a certain amount of milk. These results indicated that SDS-PAGE gel could be used to find the characteristic bands of different kinds of proteins. Based on these characteristic bands, molecular identification of different types of proteins can be carried out.
},
 year = {2023}
}

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VL - 11
IS - 6
ER -

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