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Diversity of Enset Landraces (Ensete ventricosum (Welw) Cheesman) in Aleta Chuko District, Sidama Zone, South Nation Nationality People and Regional State, Ethiopia
Amare Seifu Assefa,
Daniel Fitamo
Issue:
Volume 4, Issue 1, February 2016
Pages:
1-7
Received:
10 November 2015
Accepted:
27 November 2015
Published:
8 January 2016
DOI:
10.11648/j.jps.20160401.11
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Abstract: The objective of this study was to investigate the diversity of enset landraces in Aleta Chuko district. Accordingly, the study was conducted on the diversity of enset landraces in 125 randomly selected households at five different Kebeles (neighborhoods, the smallest administrative units in Ethiopia) in Aleta Chuko district, Southern Ethiopia. Based on the interview and filed observation 55 enset landraces from sampled households as well as additional 6 enset landraces from un-sampled households were recorded. There were variation in the diversity of enset landraces in the selected Kebeles of the district with altitudinal variation, the highest diversity being recorded in Lelawomerea with high altitude (r = 0.85, p <0.05) and lowest in Rufowayino (low altitude) with relative dissimilarity coefficient of 74%. The result from this study showed that diversity, richness, and evenness of enset landraces varied along the study Kebeles. These variations largely depend on elevation, climate, precipitation, availability of sucker, good management and presence of organic fertilizer (animal dung).
Abstract: The objective of this study was to investigate the diversity of enset landraces in Aleta Chuko district. Accordingly, the study was conducted on the diversity of enset landraces in 125 randomly selected households at five different Kebeles (neighborhoods, the smallest administrative units in Ethiopia) in Aleta Chuko district, Southern Ethiopia. Bas...
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A Glutathione S-transferase Elutes with Glyoxalase-I (Gly-I) During Purification of Gly-I from Maize (Zea mays L.) Seedlings
Md. Motiar Rohman,
Afsana Hoque Akhi,
Nusrat Jahan Methela,
Mohammad Golam Hossain,
M. Shalim Uddin,
Mohammad Amiruzzaman,
Bhagya Rani Banik
Issue:
Volume 4, Issue 1, February 2016
Pages:
8-12
Received:
27 December 2015
Accepted:
8 January 2016
Published:
21 January 2016
DOI:
10.11648/j.jps.20160401.12
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Abstract: In this study an attempt was taken to purify Glyoxalase-I (Gly-I: E.C., 4.4.1.5), from maize seedlings. Both green and non-green parts of 7 day old maize seedlings were used as plant materials. Crude proteins were precipitated by 65% (NH4)2SO4, and dialyzed overnight. The dialyzate was applied on DEAE-cellulose chromatography and eluted with linear gradient of KCl from 0 to 0.2 M. In both cases, Gly-I eluted at approximately 85 mM of KCL. The active Gly-I fractions were pooled and applied on a hydroxylapatite chromatography and eluted with 0-40 mM potassium-phosphate buffer, but the eluted fractions showed very poor activity. Therefore, the active pooled fraction of DEAE-chromatography was then applied directly on affinity chromatography (S-hexyl glutathione-agarose) for final purification and eluted with 1.2 mM of S-hexyl glutathione. The purified protein from green and non-green part had specific activity of 33.23 and 39.25 μmol min-1 mg-1 protein, respectively, along with recovery of 1.47 and 162, respectively, and yield of 83.11 and 68.15, respectively. In SDS-PAGE, the active purified affinity fraction was found to move with another protein. The spectrophotometric analysis of high active Gly-I fractions from DEAE-cellulose and affinity chromatography showed GST [another detoxifying enzyme (E.C., 2.5.1.18)] activity. This result suggested that one of the adjacent protein bands in SDS-PAGE was due to presence of a GST in Gly-I fraction.
Abstract: In this study an attempt was taken to purify Glyoxalase-I (Gly-I: E.C., 4.4.1.5), from maize seedlings. Both green and non-green parts of 7 day old maize seedlings were used as plant materials. Crude proteins were precipitated by 65% (NH4)2SO4, and dialyzed overnight. The dialyzate was applied on DEAE-cellulose chromatography and eluted with linear...
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Registration of “Ebbisa” a Newly Released Fenugreek Variety for Bale Mid Lands, Ethiopia
Mohammed Beriso,
Seifuddin Mahadi,
Yonas Worku,
Getachaw Asaffa
Issue:
Volume 4, Issue 1, February 2016
Pages:
13-16
Received:
20 December 2015
Accepted:
11 January 2016
Published:
29 February 2016
DOI:
10.11648/j.jps.20160401.13
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Abstract: Ethiopia has suitable environmental condition for fenugreek production. However, the productivity of fenugreek is very low as compared with world average due to lack of stable, high yielding, and disease resistant genotypes. Therefore, the objective of this study was to evaluate the promising genotypes and verify the most stable, high yielding and disease resistant fenugreek genotype for midlands of Bale. For the study, the experiment was carried out at three locations from 2009 to 2011production season using 16 genotypes including local and standard checks. Randomized complete block design with three replications was used for the experiment. One of the experiments was conducted at the research farm of Sinana Agricultural Research Center, Oromia Agriculture Research Institute, Sinana and the others at the farmers’ field, Goro and Ginnir. The study found that the Ebbisa (AC-TR-7) variety had above average yield performance in most test environments, out yielded the Chala and Hunda’ol varieties. The average seed yield of Ebbisa over locations and over years is about 13.8 qt/ha. It has also better yield stability than checks. Ebbisa is, therefore, released in 2012/13 for production for all fenugreek growing environments in the midlands of Bale and other similar agro-ecologies.
Abstract: Ethiopia has suitable environmental condition for fenugreek production. However, the productivity of fenugreek is very low as compared with world average due to lack of stable, high yielding, and disease resistant genotypes. Therefore, the objective of this study was to evaluate the promising genotypes and verify the most stable, high yielding and ...
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