Abstract: Waste effluents from textile industries, newspaper printing and photography contain residues of dyes and chemicals. Synthetic dyes present in water bodies, even at very low concentrations, can be extremely toxic to the living organisms due to cutting the development of bacteria and preventing the photosynthesis in aqueous flora. Acid Blue 25 (AB25) and Pigment Yellow 101 (PY101) are two types of azodyes which are toxic organic pollutants that consist of both N=N and C=C chromophoric groups. They cause considerable health effects being irritating to skin, eye and respiratory system and in some cases they may cause cancers. In this study, a biofilm of Aspergillus terreus supported on activated carbon was used as a biological tool for removal of these dyes from water body. The adsorption kinetics and contact time were determined along 240 minutes (30 minute interval). The initial concentration of dyes was 100 mg/L for each one. The biomass dosage was 1g / L. The optimum pH value for adsorption process was 3.0. The results depicted that the contact time for biosorption of both dyes was 180 min while adsorption kinetics of was quietly fitted with Pseudo second-order kinetics equation models. The maximum adsorption capacity was 82.3 and 78.2 mg/g for AB 25 and PY 101, respectively. Freundlich model was a good model for adsorption isotherm of two dyes on biofilm.Abstract: Waste effluents from textile industries, newspaper printing and photography contain residues of dyes and chemicals. Synthetic dyes present in water bodies, even at very low concentrations, can be extremely toxic to the living organisms due to cutting the development of bacteria and preventing the photosynthesis in aqueous flora. Acid Blue 25 (AB25) a...Show More