Preliminary Analysis of Sugar Supplementation on Alcoholic Fermentation by Meyerozyma guilliermondii
Gidado Rose Suniso Maxwell,
Etuk-Udo Godwin Akpan,
Olatiilu Olukemi Anna,
Isu Rosemary Nennaya,
Habu Josiah,
Solomon Bamidele Ogbe
Issue:
Volume 2, Issue 5, October 2017
Pages:
68-77
Received:
10 August 2017
Accepted:
29 August 2017
Published:
25 September 2017
Abstract: Non Saccharomyces yeast strains consume a diverse range of sugars, capable of producing ethanol at different quantities and concentrations. The ability of such wild type indigenous strains to do so and compete with industrial strains of Saccharomyces cerevisae is not common in Nigeria. This study aimed at comparing the ability of Meyerozyma guilliermondii with a strain of Saccharomyces cerevisiae, to consume sugars (fructose, galactose, glucose, lactose, sucrose and molasses) and to convert them into ethanol during fermentation. Yeast extract (6g/L), peptone (10g/L), malt extract (6g/L) broth was supplemented with different concentrations (5g/L, 10g/L, 20g/L, 30g/L) of fructose, galactose, glucose, lactose and sucrose respectively. Sugar utilization post incubation for 96 hours at 120 rpm, 30°C was measured using a refractometer. The alcoholic yield using molasses for Meyerozyma guilliermondii 9.2±0.45 (mg/ml) was significantly higher than that of Saccharomyces cerevisiae strain T (4.8±1.15 mg/ml) at 96 hours. Ethanol production from the consumption of fructose as the sole carbon source was more favourable for M. guilliermondii 2.1, 3.0, 8.11 and 9.06 (mg/ml) compared to 1.08, 3.12, 8.06 and 6.0 (mg/ml) for S. cerevisiae. Both strains displayed similar adaptation to galactose metabolism at all tested concentrations. With glucose, M. guilliermondii yielded more than its S. cerevisiae counterpart at 1.0% (4.15, 3.18 mg/ml) and 2.0% glucose (4.25, 3.3 mg/ml). At 3.0% glucose broth content, 8.15 and 9.08 mg/ml ethanol was obtained for M. guilliermondii and S. cerevisiae respectively. Sucrose utilization resulted in a 10.18 mg/ml yield of ethanol compared to a 7.06 mg/ml yield for M. guilliermondi and S. cerevisiae respectively at 3.0% sugar supplement. Meyerozyma guilliermondii displayed its ability as a highly adaptable non Saccharomyces yeast specie capable of producing ethanol from a variety of sugars indicative of local feedstock as a suitable alternative.
Abstract: Non Saccharomyces yeast strains consume a diverse range of sugars, capable of producing ethanol at different quantities and concentrations. The ability of such wild type indigenous strains to do so and compete with industrial strains of Saccharomyces cerevisae is not common in Nigeria. This study aimed at comparing the ability of Meyerozyma guillie...
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Ethnobotany of Ecological-Based Tourist Centres in the Western Zone of Ekiti State, Nigeria
Joshua Kayode,
Olatunji Akinyele,
Modupe Janet Ayeni
Issue:
Volume 2, Issue 5, October 2017
Pages:
78-86
Received:
29 July 2017
Accepted:
22 August 2017
Published:
4 October 2017
Abstract: The rapid appraisal method was used to assess the potentials of ecological-based tourism in three tourist centres situated in the western zone of Ekiti State, Nigeria. Visits were made to the centres. In each centre, the tree species, in the vegetation, situated within 50m radius of the main attractive feature of the centre were identified. The ethnobotanical values of the species were defined by 20 randomly selected aboriginal respondents that were interviewed with the aid of semi-structured questionnaire matrix. Also, in each centre, 10 tourists were randomly selected and interviewed on the values of the flora species in the centre. While the aboriginal respondents were versed on the identification and ethnobotanical values of the flora species, the tourist respondents were grossly deficient on these. Thus the results obtained revealed that the centres could serve as avenues to educate tourists on the biodiversity, ecology and ethnobotany of flora species in the immediate vicinities of the centres. Also, restoration of Nature’s Study as a subject in primary schools will also improve the consciousness and awareness of the populace on the conservation of the nation’s renewable natural resources.
Abstract: The rapid appraisal method was used to assess the potentials of ecological-based tourism in three tourist centres situated in the western zone of Ekiti State, Nigeria. Visits were made to the centres. In each centre, the tree species, in the vegetation, situated within 50m radius of the main attractive feature of the centre were identified. The eth...
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Influence of Industrial Wastewater in Douala City on Growth, Chlorophyll and Cadmium Levels of Vigna unguiculata of Economic Importance
Priso Richard Jules,
Promesse-ssie Mpahmadje Yves,
Biye Elvire Hortense,
Idole Olenguena Florentine Aimée,
Taffouo Victor Desiré,
Din Ndongo
Issue:
Volume 2, Issue 5, October 2017
Pages:
87-95
Received:
22 August 2017
Accepted:
5 September 2017
Published:
12 October 2017
Abstract: Over the world, biodiversity conservation issues related to environmental protection are vital. Douala is one of the cities where water pollution is important. Thus, a comparative study has been carried out on some growth parameters (germination, leaf surfaces, length of stem, number of leaves), chlorophyll and cadmium contents in leaves of Vigna unguiculata grown at the vicinity of three polluted water discharged sites (Azur, Industrial Zone of Bonaberi and Abattoir of Bonendale) and a non-polluted site (control). The methodology consisted of sowing cowpea in both polluted and unpolluted sites and then the growth parameters were taken every two days for six weeks during which chlorophyll in the leaves was measured after thirty days. Moreover, the degree of pollution of waste water was analyzed in the laboratory based on physicochemical parameters. The results show that plant growth is abnormal in contaminated sites (Industrial Zone of Bonaberi, Azur and Abattoir of Bonendale and the average chlorophyll (a + b) of the leaves is 36.32 for the control and 11.23; 5.26; 17.42 respectively for polluted sites (Azur, Industrial Zone of Bonabéri and Abattoir of Bonendale). These values are significantly different. Plants in the control site concentrate less Cadmium than those in polluted sites. The Cadmium maximum absorption is observed in a site located in the Industrial Zone of Bonaberi with nearly six times the value of the control.
Abstract: Over the world, biodiversity conservation issues related to environmental protection are vital. Douala is one of the cities where water pollution is important. Thus, a comparative study has been carried out on some growth parameters (germination, leaf surfaces, length of stem, number of leaves), chlorophyll and cadmium contents in leaves of Vigna u...
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