The Decoction of Cymbopogon citratus (Poaceae) Exerts Anxiolytic and Antioxidant Effects in Mice on Stress Paradigm Tests
Jean Pierre Omam Omam,
Andre Hamadou,
Antoine Kavaye Kandeda,
Juliette Koube,
Djibrilla Mana Moussa,
Brice Kevin Tchoupe Djoumsie,
Mireille Delphine Minkoulou Ze,
Roger Sébastien Ndjock,
Elisabeth Ngo Bum
Issue:
Volume 9, Issue 2, June 2023
Pages:
14-23
Received:
7 February 2023
Accepted:
1 April 2023
Published:
10 May 2023
Abstract: Cymbopogon citratus has many medicinal properties: it is used to treat headaches, cough, malaria and anxiety or fear in the national dielet. The interest of this study was to evaluate the anxiolytic properties and acute toxicity of the decoction of Cymbopogon citratus leaves. C. citratus was harvested in the Wouri division, Littoral Region of Cameroon. The identification of the families of secondary metabolites was carried out on the leaves decoction through staining and precipitation tests. The animal material consisted of male and female Mus musculus Swiss mice of 18 g at 30 g over aged about 9 weeks and not previously tested. They were used for acute toxicity assessment by administering a single dose of 5000mg/kg. Then, the anxiolytic activity assessment of the decoction on basic and chronic anxiety was done using the following devices: the elevated plus maze, the open field, the hole board and the restrictor. Finally, some oxidative stress parameters such catalase, sulphoxide dismutase, reduced glutathione and malondialdehyde were measured. The extraction yield of the C. citratus decoction was 15.12%. No signs of toxicity were observed at the dose of 5000mg/kg, 14 days after treatment. The Screening of the decoction revealed the presence of saponins, phenolic compounds and sterols. The anxiolytic activity assessment of the decoction on basic and chronic anxiety through the different devices shows that the most effective dose of the C. citratus decoction was 151 mg/kg. In the case of the elevated plus maze test, there was an increase of the number of entries into the open arms, the time spent in the open arms and their respective percentages. However, there was a decrease of the number of entries into the closed arms, the time spent in the closed arms and their percentages. The number of rearing and the mass of stool produced were significantly reduced in the maze, indicating a decrease of the level of anxiety in these mice. The open field and hole board tests also showed that, the C. citratus decoction would have anxiolytic properties. This could be justified may be by the presence of saponins and phenolic compounds. The determination of oxidative stress markers showed that, mice given the decoction and diazepam had elevated catalase, sulphoxide dismutase and reduced glutathione, but their malondialdehyde was low. The results of the study showed that the decoction of Cymbopogon citratus has anxiolytic properties.
Abstract: Cymbopogon citratus has many medicinal properties: it is used to treat headaches, cough, malaria and anxiety or fear in the national dielet. The interest of this study was to evaluate the anxiolytic properties and acute toxicity of the decoction of Cymbopogon citratus leaves. C. citratus was harvested in the Wouri division, Littoral Region of Camer...
Show More
Optimum Sterilization Department Design in Low-Medium Healthcare Facilities
Khaled Sayed Ahmed,
Fayroz Farouk Shereif
Issue:
Volume 9, Issue 2, June 2023
Pages:
24-29
Received:
21 May 2023
Accepted:
8 June 2023
Published:
21 June 2023
Abstract: Due to importance of sterilization department design and its relation to rest of sections in hospitals, selection of department location together with internal pathways are very critical issue in hospital design. To minimize the time of sterilization and comply with Infection control rules, the design of sterilization department should follow some specs and guidelines that to facilitate the operation processes and to have maximum effectiveness. In this research, some designs have been studied considering the hospital capacities (Low and medium), available space area for department, operating theater department (number of operating rooms), waste management in addition to sterilization equipment capacities that to optimize the required spaces and area that fit the needed guidelines. The results of this study are to evaluate the current designs and their relations to different departments and indicate the important points of design in addition to illustrating the optimum design for area and cost after applying logistic regression. The results reveal that hospitals with under 100 patient beds capacity and with a maximum of four operating rooms can fit their required functions and guidelines in only two sub-sections areas of CSSD in about 35-50 M2. The two sub-sections are one area for decontamination and washing and the second area is for packing and sterilization together with storage area.
Abstract: Due to importance of sterilization department design and its relation to rest of sections in hospitals, selection of department location together with internal pathways are very critical issue in hospital design. To minimize the time of sterilization and comply with Infection control rules, the design of sterilization department should follow some ...
Show More
