Molecular Diversity of Cipadessa baccifera (Roth) Miq Based on Inter Simple Sequence Repeats
E. Jebarubi,
S. Christopher Patrick Kiladi,
T. Leon Stephan Raj
Issue:
Volume 5, Issue 1, February 2017
Pages:
1-4
Received:
31 October 2016
Accepted:
15 November 2016
Published:
13 January 2017
Abstract: Medicinal plants are nature’s gifts and used in different traditional medicinal systems of India. Most of the medicinal plants are obtained from forest without proper management and knowledge of collection. For the authentication of medicinal plants from the substituted materials, a genetic diversity study is necessary. In the present investigation molecular diversity of Cipadessa baccifera was carried out using dominant ISSR (inter simple sequence repeats) markers in four different accessions of Western Ghats of South India. Five primers were selected from a total of ten primers based on the reproducibility of the bands. The number of polymorphic loci was 13 and the percentage of polymorphic loci was 56.52. The genetic distance among the populations ranged from 0.0910 to 0.3629 and the genetic identity ranged from 0.6087 to 0.9130. The overall observed and effective number of alleles was about 1.5652 and 1.3913. Nei’s overall genetic diversity and Shannon information index were 0.2283 and 0.3349. The dendrogram was constructed based on the UPGMA method and the clusters formed depending upon the genetic characters.
Abstract: Medicinal plants are nature’s gifts and used in different traditional medicinal systems of India. Most of the medicinal plants are obtained from forest without proper management and knowledge of collection. For the authentication of medicinal plants from the substituted materials, a genetic diversity study is necessary. In the present investigation...
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A Comprehensive Physical, Spectroscopic, and Thermal Characterization of Withania somnifera (Ashwagandha) Root Extract Treated with the Energy of Consciousness (The Trivedi Effect®)
Mahendra Kumar Trivedi,
Alice Branton,
Dahryn Trivedi,
Gopal Nayak,
Cathryn Dawn Nykvist,
Celine Lavelle,
Daniel Paul Przybylski,
Dianne Heather Vincent,
Dorothy Felger,
Douglas Jay Konersman,
Elizabeth Ann Feeney,
Jay Anthony Prague,
Joanne Lydia Starodub,
Karan Rasdan,
Karen Mie Strassman,
Leonid Soboleff,
Maire Anne Mayne,
Mary M. Keesee,
Padmanabha Narayana Pillai,
Pamela Clarkson Ansley,
Ronald David Schmitz,
Sharyn Marie Sodomora,
Kalyan Kumar Sethi,
Parthasarathi Panda,
Snehasis Jana
Issue:
Volume 5, Issue 1, February 2017
Pages:
5-14
Received:
6 February 2017
Accepted:
18 February 2017
Published:
7 March 2017
Abstract: Withania somnifera (Ashwagandha) root extract possesses a broad range of pharmacological activities. The aim of current study was to explore the impact of The Trivedi Effect® - Energy of Consciousness Healing Treatment (Biofield Energy Healing Treatment) on the physical, spectroscopic, and thermal properties of ashwagandha root extract using PXRD, PSD, FT-IR, UV-vis spectroscopy, TGA, and DSC analysis. Ashwagandha root extract was divided into two parts. One part was denoted as the control, while the other part was defined as The Trivedi Effect® - Biofield Energy Treated sample, which received The Trivedi Effect® Treatment remotely from eighteen renowned Biofield Energy Healers. The PXRD analysis exhibited that both the control and The Trivedi Effect® - Biofield Energy Treated samples were amorphous in nature. The particle size values at d10 and d50 of the treated sample were decreased by 4.66% and 6.02% and at d90 slightly increased by 0.99% compared with the control sample. The average surface area of the treated (0.130 m2/g) sample was increased by 4.84% compared with the control (0.124 m2/g) sample. The FT-IR results suggested that the force constant of O-H (stretching), and C-O (stretching) functional groups of the treated sample were reduced compared with the control sample. The UV-vis analysis revealed that the wavelength for the maximum absorbance of the control and treated samples were at 205.4 nm and 205.6 nm, respectively. The TGA thermograms exhibited three steps of the degradation process, and the total weight loss of the treated (86.45%) sample was increased by 1.44% compared with the control (85.22%) sample. The DSC analysis revealed that melting point of the treated (201.67°C) sample was reduced by 0.87% compared with the control (203.43°C) sample. The latent heat of fusion of the treated (6.77 J/g) sample was significantly decreased by 21.82% compared to the control (8.67 J/g) sample. Hence, the treated sample would be more advantageous for the better therapeutic responses compared to the control sample. These findings suggest that The Trivedi Effect® - Energy of Consciousness Healing Treatment might have the astounding capacity to enhance the solubility, dissolution, absorption, and bioavailability of ashwagandha root extract in various forms of pharmaceutical/nutraceutical formulations by modifying its particle size and surface area. Thus, The Trivedi Effect® - Energy of Consciousness Healing Treated ashwagandha root extract might provide better therapeutic response against inflammatory diseases, immunological disorders, stress, arthritis, cancer, diabetes, sexual disorders, aging and other chronic infections.
Abstract: Withania somnifera (Ashwagandha) root extract possesses a broad range of pharmacological activities. The aim of current study was to explore the impact of The Trivedi Effect® - Energy of Consciousness Healing Treatment (Biofield Energy Healing Treatment) on the physical, spectroscopic, and thermal properties of ashwagandha root extract using PXRD, ...
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