Research Article
Isolation, Identification and Antibiotics Susceptibility Test of Escherichia coli from Abattoir and Butchers in Bishoftu Town
Ephrem Shimelis*
,
Muluken Tekle,
Serkaddis Alem
Issue:
Volume 14, Issue 1, February 2026
Pages:
1-7
Received:
22 March 2026
Accepted:
6 May 2026
Published:
19 May 2026
Abstract: E. coli with multiple antibiotics resistance is one of the public health issues concerned with the Food of animal origin safety. To control this pathogen; isolation and determining of antimicrobial resistance is a key tool. Therefore, the objective of the current study was to isolate, identify and detect the antimicrobial resistance of E. coli isolated from meat sample collected from selected slaughter house and Butchers in Bishoftu town central Ethiopia. Purposive sampling techniques were applied to collect 80 samples from which 47 Carcass swab. 12 material swabs and 21 carcasses from the selected abattoir (32) and Butchers (48). Conventional bacteriological laboratory culturing, on Eosin-Methylene blue to isolate general E. coli and on Sorbitol MacConkey agar to isolated pathogenic E. coli from general E. coli and biochemical test for E. coli identification and Disc diffusion antimicrobial susceptibility test were applied to isolate The E. coli. Out of 80 samples tested 18.75% of the samples were positive for the occurrence of the E. coli: The incidence of E. coli occurrence in two different meat value chain (Abattoir and Butcher) indicated the frequency of 25% and 14.5% respectively with no significant difference between them (P-value > 0.05). For antimicrobial sensitivity test out of eleven (11) antibiotics disk selected, two drugs, namely; Ampicillin and penicillin were 100% resistant to fifteen E. coli isolates; while fifteen isolates of E. coli were 100% susceptible to Gentamycin and sulphametazole. The antibiotic resistant test result in current study indicated that each E. coli isolate was resistant to at least two antibiotics. Therefore, Good hygienic and safety practice should be applied both at slaughter house and butchers, awareness creation among the society about the risk of transmission, prevention and controls of multidrug resistant E. coli should be given.
Abstract: E. coli with multiple antibiotics resistance is one of the public health issues concerned with the Food of animal origin safety. To control this pathogen; isolation and determining of antimicrobial resistance is a key tool. Therefore, the objective of the current study was to isolate, identify and detect the antimicrobial resistance of E. coli isol...
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Research Article
Screening of Sorghum Genotypes Resistance to Sorghum Shoot Fly (Atherigona Soccata) In West Hararghe, Oromia
Daba Etafa*
Issue:
Volume 14, Issue 1, February 2026
Pages:
8-13
Received:
27 April 2026
Accepted:
4 June 2026
Published:
25 June 2026
DOI:
10.11648/j.bio.20261401.12
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Abstract: Shoot fly (Atherigona soccata) is one of the major insect pests limiting sorghum production by causing severe seedling damage and significant yield losses. Although management options such as timely planting, seed treatment with systemic insecticides, and foliar insecticide applications are available, their effectiveness is often constrained by rainfall variability and the high cost of insecticides for resource-poor farmers. Therefore, host plant resistance offers an economically viable and environmentally sustainable approach for managing shoot fly infestations. This study aimed to identify sorghum genotypes with stable resistance to shoot fly under field conditions. The experiment was conducted at the Mechara Agricultural Research Center (McARC), Ethiopia, where eighty sorghum genotypes were evaluated for their response to shoot fly infestation. The results revealed substantial variation among genotypes in their level of resistance. Out of the eighty genotypes tested, fifty-nine exhibited resistant or tolerant reactions to shoot fly damage. These resistant/tolerant genotypes represent valuable genetic resources for sorghum improvement programs and can be utilized in future breeding efforts aimed at developing shoot fly-resistant varieties. The identified genotypes provide a sustainable option for enhancing sorghum productivity and reducing yield losses caused by shoot fly infestations.
Abstract: Shoot fly (Atherigona soccata) is one of the major insect pests limiting sorghum production by causing severe seedling damage and significant yield losses. Although management options such as timely planting, seed treatment with systemic insecticides, and foliar insecticide applications are available, their effectiveness is often constrained by rai...
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