Role of microRNA-33a/b in Cholesterol Metabolism in Type 2 Diabetic Patients in Ouagadougou, Burkina Faso
Alice Kiba Koumaré,
Tegwinde Rebeca Compaoré,
Fabienne Soudré,
Raoul Karfo,
Gnabôrou Rachid Konfé,
Elie Kabré,
Ignatius Baldeh,
Jacques Simporé,
Jean Sakandé
Issue:
Volume 7, Issue 4, December 2019
Pages:
71-76
Received:
8 December 2019
Accepted:
21 December 2019
Published:
4 January 2020
Abstract: MicroRNAs (miRNAs) are small endogenous RNAs approximately 22 nucleotides involved in the regulation of several cellular metabolisms including cholesterol metabolism. The objective of this study was to measure miRNAs 33a and 33b in type 2 diabetics to evaluate their impact on the lipids levels and prevalence of dyslipidemia. The study population profile was 45 subjects including 30 type 2 diabetic patients and 15 healthy controls. The lipids tests were performed using an automated Spintech 240 Biolis analyzer and the microRNAs (33a and 33b) by applied biosystems 7500 Fast Real Time PCR System using the TaqMan® MicroRNA Assay kit. The prevalence of dyslipidemia was higher in miRNA-33a positive subjects than miRNA-33a negative (p <0.0001). The prevalence of dyslipidemia was however not significant between miRNA-33b positive and miRNA-33b negative. A comparison between miRNA-33a positive and miRNA-33b positive showed a significant increase of dyslipidemia in miRNA-33a positive than in miRNA-33b positive subjects. The dyslipidemic types in miRNA-33a positive diabetics were 90% hypercholesterolemia, 88% LDLC increase and 83.33% HDLC decrease. The measurement of the HDLC subclasses showed 82.6% HDL2C decrease and 90.91% HDL3C increase. The HDL3C level increased in 100% of non hypertensive diabetics versus 46.67% in hypertensive diabetics (p=0.003). The increase of HDL3C was 90.9% in miRNA33a positive subjects versus 54.5% in miRNA33b positive subjects (p <0.006). The study therefore confirms the relationship between the presence of microRNAs 33a and increased cardiovascular risk. The results showed a role of microRNA-33a on the increase of HDL3C which has a weak atheroprotective role compared to HDL2C. This observation suggests that the research on drugs able to increase the HDLC level based on microRNA regulation should target the stimulation of HDL2C synthesis.
Abstract: MicroRNAs (miRNAs) are small endogenous RNAs approximately 22 nucleotides involved in the regulation of several cellular metabolisms including cholesterol metabolism. The objective of this study was to measure miRNAs 33a and 33b in type 2 diabetics to evaluate their impact on the lipids levels and prevalence of dyslipidemia. The study population pr...
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Problems in the Research of Meliboeus ohbayashii primoriensis (Coleoptera: Biprestidae)
Cui Yaqin,
Liu Suicun,
Sun Yongming,
Yao Limin
Issue:
Volume 7, Issue 4, December 2019
Pages:
77-81
Received:
21 November 2018
Accepted:
6 December 2018
Published:
8 January 2020
Abstract: At present, a series of researches on Meliboeus ohbayashii primoriensis mainly focus on the basic research of bioecology and multi-control methods, whereas, some problems still exist in the researches. In order to provide a better theoretical basis and scientific basis for comprehensive control for M. ohbayashii primoriensis. Based on referring to research literature of M. ohbayashii primoriensis, including the research status, existing problems and control technology. It has been clear about the host plants, scientific name correction and control method for M. ohbayashii primoriensis. It can provide guidance on the strong theoretical basis, and scientific foundation for the integrated control of walnut insect pest.
Abstract: At present, a series of researches on Meliboeus ohbayashii primoriensis mainly focus on the basic research of bioecology and multi-control methods, whereas, some problems still exist in the researches. In order to provide a better theoretical basis and scientific basis for comprehensive control for M. ohbayashii primoriensis. Based on referring to ...
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